Sable (Martes zibellina), belongs to Carnivora, Mustelidae and Maretes, was mainly distributed among the cold northern zone of Eurasia. The purpose of this study is to explore the intestinal flora of the sable by the method of the metagenomic library-based technique, libraries were sequenced on an Illumina HiSeq 4000 instrument. Effective Data volume of each sample is above 6000M, the ratio of the Effective Data (the Clean Data) to original Data (Raw Data) is over 98%. According to the analysis of statistical data, the Total length of ORF is about 603,031, which is 347.36 Mbp. We contrast the unique function of genes with KEGG database, we acquire 7140 genes (KO), a total of all the samples KO is 129788. We selected higher abundance genes to draw cluster heat maps, and according to the results of the KEGG metabolic pathway annotations, we acquire the gene function,including metabolism, environmental information processing, genetic information processing, cellular process and organismal systems. We contrast the unique function of genes with CAZy database, the functional carbohydrate hydrolases have corresponding genes in the intestinal microorganisms of the sable. This is closely related to the fact that the sable is adapted to cold environments and requires a large amount of energy to maintain its metabolic activity. We contrast the unique function of genes with eggNOG database,the main functions of genes included gene duplication, recombination and repair, transport and metabolism of amino acids, transport and metabolism of carbohydrates, etc. In this study, we intended to identify the complex microbial population structure of sables based on metagenomic sequencing method, which uses the whole metagenomic data, mapping the sequences to the known genes or the pathways in the existing databases, such as CAZy, KEGG, or eggNOG, and then exploring the genetic composition and functional diversity of microbial community based on the mapped functional categories.
The yellow-throated marten (Martes flavigula) is a medium-sized carnivore that is widely distributed across much of Asia and occupies an extensive variety of habitats. We reported a high-quality genome assembly of this organism that was generated using Oxford Nanopore and Hi-C technologies. The final genome sequences contained 215 contigs with a total size of 2,449.15 Mb and a contig N50 length of 68.60 Mb. Using Hi-C analysis, 2,419.20 Mb (98.78%) of the assembled sequences were anchored onto 21 linkage groups. Merqury evaluation suggested that the genome was 94.95% complete with a QV value of 43.75. Additionally, the genome was found to comprise approximately 39.74% repeat sequences, of which long interspersed elements (LINE) that accounted for 26.13% of the entire genome, were the most abundant. Of the 20,464 protein-coding genes, prediction and functional annotation was successfully performed for 20,322 (99.31%) genes. The high-quality, chromosome-level genome of the marten reported in this study will serve as a reference for future studies on genetic diversity, evolution, and conservation biology.
Sable (Martes zibellina), belongs to Carnivora, Mustelidae and Maretes, was mainly distributed among the cold northern zone of Eurasia. The purpose of this study is to explore the intestinal flora of the sable by the method of the metagenomic library-based technique, libraries were sequenced on an Illumina HiSeq 4000 instrument. Effective Data volume of each sample is above 6000M, the ratio of the Effective Data (the Clean Data) to original Data (Raw Data) is over 98%. According to the analysis of statistical data, the Total length of ORF is about 603,031, which is 347.36 Mbp. We contrast the unique function of genes with KEGG database, we acquire 7140 genes (KO), a total of all the samples KO is 129788. We selected higher abundance genes to draw cluster heat maps, and according to the results of the KEGG metabolic pathway annotations, we acquire the gene function,including metabolism, environmental information processing, genetic information processing, cellular process and organismal systems. We contrast the unique function of genes with CAZy database, the functional carbohydrate hydrolases have corresponding genes in the intestinal microorganisms of the sable. This is closely related to the fact that the sable is adapted to cold environments and requires a large amount of energy to maintain its metabolic activity. We contrast the unique function of genes with eggNOG database,the main functions of genes included gene duplication, recombination and repair, transport and metabolism of amino acids, transport and metabolism of carbohydrates, etc.
Sable (Martes zibellina), a member of family Mustelidae, order Carnivora, is primarily distributed in the cold northern zone of Eurasia. The purpose of this study was to explore the intestinal flora of the sable by metagenomic library-based techniques. Libraries were sequenced on an Illumina HiSeq 4000 instrument. The effective sequencing data of each sample was above 6,000 M, and the ratio of clean reads to raw reads was over 98%. The total ORF length was approximately 603,031, equivalent to 347.36 Mbp. We investigated gene functions with the KEGG database and identified 7,140 KEGG ortholog (KO) groups comprising 129,788 genes across all of the samples. We selected a subset of genes with the highest abundances to construct cluster heat maps. From the results of the KEGG metabolic pathway annotations, we acquired information on gene functions, as represented by the categories of metabolism, environmental information processing, genetic information processing, cellular processes and organismal systems. We then investigated gene function with the CAZy database and identified functional carbohydrate hydrolases corresponding to genes in the intestinal microorganisms of sable. This finding is consistent with the fact that the sable is adapted to cold environments and requires a large amount of energy to maintain its metabolic activity. We also investigated gene functions with the eggNOG database; the main functions of genes included gene duplication, recombination and repair, transport and metabolism of amino acids, and transport and metabolism of carbohydrates. In this study, we attempted to identify the complex structure of the microbial population of sable based on metagenomic sequencing methods, which use whole metagenomic data, and to map the obtained sequences to known genes or pathways in existing databases, such as CAZy, KEGG, and eggNOG. We then explored the genetic composition and functional diversity of the microbial community based on the mapped functional categories.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.