In this study, 3DPT showed great clinical feasibility of the treatment of complicated PHFs. The 3D-print PHF model had the ability to clearly display the fracture and thus was useful to determine the fracture classification and the magnitude of fracture injury. It benefited surgeons to gain a better understanding of complicated PHFs, design a most suitable operation plan prior to surgery and facilitate the doctor-patient communication. This therefore enabled the reduction of intraoperative injury and the optimization of surgical outcomes.
Background: Postmenopausal osteoporosis, a common yet chronic systemic metabolic disease, has become a major public health problem due to life expectancy increasing around the world. The differentiation of mesenchymal stem cells (MSCs) into osteoblasts, and the differentiation of circulating monocyte cells into osteoclasts, play an important role in the balance of bone metabolism. However, when both undergo pathological changes, it can lead to abnormalities, resulting in osteoporosis. This study aims to explore a new biomarker for postmenopausal osteoporosis, thereby providing a new entry point for bioinformatic research into the clinical diagnosis and treatment of the disease.Methods: Using the Gene Expression Omnibus (GEO) database, microarray analysis was conducted to identify differentially expressed genes in MSCs and monocytes in both postmenopausal osteoporosis patients and a healthy control group. The Database for Annotation, Visualization and Integrated Discovery (DAVID) database was used to analyze the function and enrichment of the selected genes, and a protein-protein interaction (PPI) network was constructed from the Search Tool for the Retrieval of Interacting Genes/ Proteins (STRING) website and displayed in Cytoscape. To achieve the final results, module analysis of the PPI network was performed by using Molecular Complex Detection (MCODE).Results: We identified 45 high-expression and 26 low-expression genes through the study, all of which underwent pathway enrichment analysis. This enrichment was observed in the cell cycle regulation, osteoclast differentiation, tumor necrosis factor (TNF) signaling pathway, and RNA transport. The top 10 hub genes of the PPI network were SF3B1,
Background: The precise pathogenesis of ankylosing spondylitis (AS) is still largely unknown at present. Our previous study found that toll-like receptor 4 (TLR4) downregulated and performed immunoregulatory dysfunction in mesenchymal stem cells from AS patients (AS-MSCs). The aim of this study was to explore the expression profiles of long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in TLR4primed AS-MSCs, and to clarify the potential mechanisms. Methods:The immunoregulatory effects of MSCs were determined after TLR4 activation. Next, the differentially-expressed (DE) lncRNAs and mRNAs between AS-MSCs and TLR4-primed AS-MSCs [stimulated by lipopolysaccharide (LPS)] were identified via high-throughput sequencing followed by quantitative real-time PCR (qRT-PCR) confirmation. Finally, bioinformatics analyses were performed to identify the critical biological functions, signaling pathways, and associated functional networks involved in the TLR4-primed immunoregulatory function of AS-MSCs.Results: A total of 147 DE lncRNAs and 698 DE mRNAs were identified between TLR4-primed AS-MSCs and unstimulated AS-MSCs. Of these, 107 lncRNAs were upregulated and 40 were downregulated (fold change ≥2, P<0.05), while 504 mRNAs were upregulated and 194 were downregulated (fold change ≥2, P<0.05). Five lncRNAs and five mRNAs with the largest fold changes were respectively verified by qRT-PCR. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses demonstrated that the DE mRNAs and lncRNAs were highly associated with the inflammatory response, such as NODlike receptor (NLR) signaling pathway, the TNF signaling pathway and the NF-κB signaling pathway. Cisregulation prediction revealed eight novel lncRNAs, while trans-regulation prediction revealed 15 lncRNAs, respectively. Eight core pairs of lncRNA and target mRNA in the lncRNA-transcription factor (TF)-mRNA network were as follows:
Introduction Upper lumbar burst fractures (L1or L2) are often followed by bilateral pedicle screw fixation at the level of fracture using posterior short-segment pedicle instrumentation. However, it can aggravate the trauma to the fractured vertebra. We have introduced a modified technique of posterior short-segment instrumentation for the treatment of upper lumbar burst fracture. The aim of this study was to compare the clinical and radiologic results of modified technique versus conventional technique using posterior short-segment pedicle instrumentation in the treatment of upper lumbar burst fractures. Methods The data from 64 patients with upper lumbar burst fracture who had undergone posterior short-segment instrumentation from April 2014 to November 2018 in our clinic were evaluated in the present retrospective study. All the patients were divided into 2 groups according to the surgical technique, including 27 patients (modified order of intraoperative pedicle screw placement) in modified group and 37 patients (conventional order of intraoperative pedicle screw placement) in conventional group. The clinical outcomes and radiological parameters were evaluated preoperatively, postoperatively, at 3-month follow-up and final follow-up.Results Technical success was achieved in all 64 patients. The operation time of modified group(130.4±32.4min) is significantly longer than conventional group (115.3±26.8min, p<0.05). Significant improvement in the anterior vertebral height(AVH) ratio (97.9%±6.7% in modified group, 94.1%±7.8% in conventional group, p<0.05) was found postoperatively, (100.1%±9.7% in modified group, 89.6%±6.7% in conventional group, p<0.01) at the 3-month follow-up and(98.8%±7.7% in modified group, 90.9%±7.6% in conventional group, p<0.01) at the final follow-up. And post-operative correction of AVH ratio was significantly better in modified group (45.0%) than in conventional group (38.8%, p<0.01). There was 1 case of wound infection in both groups respectively. No instrument loosening or failure, or breakage was observed during follow-up.Conclusions Modified technique and conventional technique of posterior short-segment pedicle screw fixation for upper lumbar burst fractures both provided immediate stability and reduction of post-traumatic segmental kyphosis. In addition, modified technique of posterior short-segment pedicle screw fixation seems to be a promising method for upper lumbar burst fractures because it led to better reduction of fractured vertebra than in patients who received conventional technique.
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