Significance and Impact of the Study: We investigated the intestinal microbiota (IM) of Quasipaa spinosa tadpoles at four key stages during metamorphosis using high-throughput sequencing of the 16S rRNA gene. Our results showed that the composition of the IM at different development stages and different ages differed markedly, but the differences were not significant among different development stages at 150 days post-hatch. These results indicate that the developmental stage and length of development significantly affect the IM composition of Q. spinosa.
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