No abstract

No abstract

Circular RNAs are a large class of noncoding RNAs. Smad5 functions in cell differentiation, cell proliferation and metastasis. It has been reported that lnc-Smad5 can inhibit the proliferation of diffuse large B cell lymphoma. However, the function of circ-Smad5 has not yet been reported. Lentivirus vectors were constructed to establish circ-Smad5 upregulated and circ-Smad5 downregulated cell models. A CCK-8 assay was used to detect the proliferation of JB6 cells. FACS was used to analyze the cell cycle in the cell models. Western blot, immunofluorescence staining and TOP/FOP flash dual luciferase activity assays were used to determine the activity of the Wnt signaling pathway. The results revealed that the expression level of circ-Smad5 in JB6 cells was significantly lower than the expression level of linearized-Smad5. Compared with the control group, the percentage of S phase cells and the expression level of cyclin D1 protein were significantly higher in the sh-circ-Smad5 group. In the sh-circ-Smad5 group, β-catenin and LEF-1 were significantly increased, p-β-catenin was significantly decreased, and the relative activity of the TOP/FOP reporter gene was higher compared to the control group levels. These phenomena could be reversed by treating with Wnt signaling inhibitor PNU-74654. We conclude that the circ-Smad5 retards the proliferation and the cell cycle progression of JB6 cells. Thus, circ-Smad5 may function by inhibiting the activation of Wnt/β-catenin/Lef 1 signaling, which inhibits the expression of cyclin D1. To the best of our knowledge, we are the first to report the function of circ-Smad5.

Estimating the impact of economic policy uncertainty (EPU) on carbon emissions is crucial for formulating emission reduction targets and policies. Using the unbalanced panel data of 325 prefecture-level cities in China from 2001 to 2017 and a two-way fixed-effects model, this paper investigates the impact of economic policy uncertainty on city’s carbon emission intensity. We find that one percentage point increase in economic policy uncertainty will make the city’s carbon emission intensity increase by 4.28 percentage points, and by 0.244 tons per ten thousand yuan at an absolute level. The findings imply that policy makers need to consider the potential threat of economic policy uncertainty on carbon peaking and carbon neutrality in China.

No abstract

No abstract

No abstract

In recent years, the research of SMEs’ eco-innovation (EI) has gained attention on an international scale. However, only a few studies provide a global research panorama. This study attempts to bridge this knowledge gap by utilizing the scientometric tools. A total of 749 articles from ISI web of science core collection were collected and analyzed to generate the result, which demonstrated a wealth of useful new conclusions in this domain. The results show that (i) the research topics on SMEs’ EI are changing dynamically and become more diversified from 1997 to 2021, especially from 2014 to 2021. (ii) The newest research frontiers are logically consistent with the previous knowledge base, while reflect the novelty of the research, which shows more correlation and adaptation to the newest development situation. (iii) The innovation paradigm in this field has not yet formed, and correlational studies are still in the early stage and a paradigm of open innovation is strongly recommended for researchers. And the barriers, category, process, outcome, and more similar subdivided themes of SMEs’ EI are worthy to be further explored. For research methods, inter-regional, inter-institutional, and interdisciplinary perspectives are hopeful to contribute to future research avenues. Some useful inspirations are also provided for practitioners to develop SMEs’ EI.