With the decline in coastal fishery resources, hatchery-reared fry of yellowfin bream (Acanthopagrus latus) have been commonly mass released to the surrounding waters in China in recent years. Although the release plan has been underway in China for many years, the releasing effects were not often assessed. Therefore, it is necessary to study several suitable mass marking methods that have a high mark retention rate for fry. From October to November 2020, tetracycline was immersed to mark calcified hard tissue (MI), with tagging cut tail fin (TC) and the control, and the species of yellowfin bream by live fish were examined. Moreover, a double marking method, i.e., mark calcified hard tissue (MI) after tag fish for two weeks, was examined to determine any increase in the mark retention rate for yellowfin bream in November 2020. The dual marking and tagging method combining MI and TC is effective and is suitable for yellowfin bream. Both MI of 0.25% treatment and MI of 0.5% treatment also have lower costs, and more individuals can be marked.
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