Long non‐coding RNA (lncRNA) urothelial carcinoma‐associated 1 (UCA1) has been identified as an oncogene and is involved in acute myeloid leukaemia (AML). Autophagy contributes to tumourigenesis and cancer cell survival. The purpose of this study was to investigate the regulatory role and mechanism of UCA1 in AML cell viability by its effect on autophagy. The expression of UCA1, miR‐96‐5p, and ATG7 was determined by qRT‐PCR and western blot. Cell proliferation was examined by MTT assay. The autophagy level was assessed by green fluorescent protein (GFP)‐LC3 immunofluorescence and western blot. The interaction between UCA1 and miR‐96‐5p or ATG7 was analyzed by luciferase reporter activity. The results showed that UCA1 promoted AML cell proliferation by inducing autophagy. Mechanistically, UCA1 acted as a sponge of miR‐96‐5p by binding to miR‐96‐5p. ATG7 was a direct target of miR‐96‐5p and positively regulated by UCA1. Further results showed that the miR‐96‐5p mimic effectively counteracted the UCA1 overexpression‐mediated induction of the ATG7/autophagy pathway. Collectively, UCA1 functions as a sponge of miR‐96‐5p to upregulate its target ATG7, thereby resulting in autophagy induction. Our findings reveal a UCA1‐mediated molecular mechanism responsible for autophagy induction in AML and help to improve the understanding of the molecular mechanism of AML progression.
Carbon molecular sieve membranes were prepared by pyrolysis of novolac type phenol-formaldehyde resin. The influences of pyrolysis temperature on membrane properties were investigated. By raising the pyrolysis temperature from 600 oC to 700 oC, the number of pores and effective pore size increased, thereby making the carbon membrane more productive but less selective. When the pyrolysis temperature from 700 oC to 900 oC, the effective pore size was reduced by sinter effect, thereby the gas permeation rate decreased and selectivity increased. The carbon membranes were characterized by elemental analysis, X-ray diffraction (XRD), and CO2 adsorption. H2, N2, CH4, and O2 were used for pure gas tests to evaluate membrane performance.
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