Unlike the better-studied aberrant epigenome in the tumor, the clinicopathologic impact of DNA methylation in the tumor microenvironment (TME), especially the contribution from cancer-associated fibroblast (CAF), remains elusive. CAFs exhibit profound patient-to-patient tumorigenic heterogeneity. We ask whether such heterogeneity may be exploited to quantify the level of TME malignancy or not. We developed a robust and efficient methylome/transcriptome co-analysis system for CAFs and paired normal fibroblasts (NFs) from non-small-cell lung cancer patients. We found 14,781 CpG sites of CAF/NF differential methylation, of which 3,707 sites showed higher methylation changes in ever-smokers than in non-smokers. Concomitant CAF/NF differential gene expression analysis pinpointed to a subset of 54 smoking-associated CpG sites with strong methylation-regulated gene expression.A methylation index that summarizes the beta-values of these CpGs was built for NF/CAF discrimination (MIND) with high sensitivity and specificity. The potential of MIND in detecting pre-malignancy across individual patients was shown. MIND succeeded in predicting tumor recurrence in multiple lung cancer cohorts without reliance on patient survival data, suggesting that the malignancy level of TME may be effectively graded by this index. Precision TME grading may provide additional pathological information to guide cancer prognosis and open up more options in personalized medicine.
PVDF-HFP nanofibers are reinforced by (0, 10, 16 and 30wt% ) of butadiene rubber (BR) and are crosslinked by the UV curing during the electrospinning process. These electrospun clothes are soaked with 70wt% uptake of EMI-Tf ionic liquid for solid state electrolytes applications. These electrolytes are investigated by a broadband dielectric spectroscopy over a wide temperature range from-40 to 80 OC. It is found that the temperature dependence of conductivity of all the samples not only follow the VFT relation but also follow the Debye-Stokes-Einstein relation implying the coupling between the ionic conduction and the segmental motion of the polymer matrix. Also, the results indicated that the addition of butadiene rubber did not significantly affect the conduction behavior of the electrolyte. Moreover, the conductivity of all the electrospun cloth electrolytes is much higher than that of the solution casting film.
Based on the experience of earthquake, through the search and summary on natural features of the hilly cities , aim at the common problem exist in disaster prevent in urban planning and lost in research, research on the specific construction model and technical methods in four-dimensional disaster prevention space of the hilly cities which possess dynamic and comprehensive.
The paper introduced the basic performance and thermal insulation mechanism of a new building thermal insulation material—FHP-Vc inorganic composite silicate heat-insulating board which is used as the thermal insulation material for exterior wall. Based on the experiments and analysis of the heat transfer performance of FHP-Vc board, it has been found that the thermal performance of FHP-Vc board meets the current design standard of building energy efficiency. At the same time, it is a new building energy-saving material which has good insulation, non-burning, reliable and economical properties.
This article introduces the method of the building enclosure construction of energy saving residential buildings based on the FHP-Vc inorganic composite silicate heat-insulating board and its practical effect in projects.
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