By integrating imaging and drug-delivery in a single system, fluorescent nano-multifunctional imaging platforms can offer simultaneous diagnosis and therapy to diseases like cancer. However, the synthesis of such system involves a tedious, time-consuming, and multi-step process. Herein we report a facile method based on simple ultrasonication to synthesize highly cross-linked, monodispersed fluorescent polyphosphazene nanoparticles from hexachlorocyclotriphosphazene (HCCP) and dichlorofluorescein (FD). Various functional groups (folic acid, PEG-NH, and methylene blue) can be "fastened" in situ onto the poly(cyclotriphosphazene-co-dichlorofluorescein) (PCTPDF) nanoparticles to expand its application as nano-multifunctional platform. All the nanoparticles were characterized spectrophotometrically, and morphology was established by the images obtained from scanning electron microscope (SEM). The synthesized multifunctional nanoparticles exhibited low toxicity and penetrated through the cytomembranes of human colon cancer (HCT 116) cells. When applied to in vivo tumor imaging using biologically engineered mouse model, methylene blue functionalized (PCTPDF@MB) nanoparticles exhibited excellent photodynamic activity and imaging ability. Thus, PCTPDF nanoplatform based on multi-functional fluorescent nanoparticles might offer an efficient solution to new age theranostics. Apart from diagnostics application, PCTPDF, as a nanoplatform, could also be utilized to achieve more comprehensive application in modern analytic chemistry. Graphical Abstract The table of contents.
The
basic liquid–liquid equilibrium data of two systems
{water + 2-propanol (IPA) + 1-octanol/n-propyl acetate}
were measured. The distribution coefficients of the 2-propanol and
the separation factors of the systems were calculated to analyze the
extraction ability of the two extractants that extract IPA from water.
The correlation model Hand equation was used to fit the data. The
value of the linear correlation coefficient R
2 > 0.99 indicates that the linear fit of the experimental
data is good. The theoretical models NRTL and UNIQUAC were used to
correlate the measured data. The deviation between experimental data
and simulated data was calculated by the root mean square deviation
(RMSD). The RMSD < 0.01 means that the two models were suitable
for the regression of the systems studied in this work. GUI-MATLAB
has validated the binary interaction parameters. The basic data obtained
from this work can support the separation of water and IPA.
The
liquid–liquid equilibrium (LLE) data of water + furfuryl
alcohol + trichloromethane /methyl isopropyl ketone were determined
at 303.2, 313.2, and 323.2 K under 101.3 kPa. The distribution coefficient
(D) and separation factor (S) were
evaluated for the separation effect of solvents. The nonrandom two-liquid
and universal quasichemical models were used to correlate the experimental
LLE data and obtain the binary interaction parameters. Meanwhile,
the root mean square deviations and absolute average deviation of
the two models did not exceed 0.0094 and 0.0066, respectively, which
shows that the experimental LLE data can be accurately correlated
through the two models. GUI-MATLAB was used to verify the consistency
between the LLE data and the calculated binary interaction parameters.
σ-Profiles were used to analyze the interaction between the
molecules of each substance, further confirming the accuracy of the
experimental results.
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