There are two proposed explanations for ultraluminous X-ray sources (ULXs) with luminosities in excess of 10(39) erg s(-1). They could be intermediate-mass black holes (more than 100-1,000 solar masses, M sun symbol) radiating at sub-maximal (sub-Eddington) rates, as in Galactic black-hole X-ray binaries but with larger, cooler accretion disks. Alternatively, they could be stellar-mass black holes radiating at Eddington or super-Eddington rates. On its discovery, M 101 ULX-1 had a luminosity of 3 × 10(39) erg s(-1) and a supersoft thermal disk spectrum with an exceptionally low temperature--uncomplicated by photons energized by a corona of hot electrons--more consistent with the expected appearance of an accreting intermediate-mass black hole. Here we report optical spectroscopic monitoring of M 101 ULX-1. We confirm the previous suggestion that the system contains a Wolf-Rayet star, and reveal that the orbital period is 8.2 days. The black hole has a minimum mass of 5 M sun symbol, and more probably a mass of 20 M sun symbol-30 M sun symbol, but we argue that it is very unlikely to be an intermediate-mass black hole. Therefore, its exceptionally soft spectra at high Eddington ratios violate the expectations for accretion onto stellar-mass black holes. Accretion must occur from captured stellar wind, which has hitherto been thought to be so inefficient that it could not power an ultraluminous source.

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The vibration-stress field could be formed by high vibration intensity in vibration machine to improve the ability of the collision, shock, shear and extrusion for the system, and it also can avoid plugging screen for vibration screening machine, which could make for solving some special requirements of the vibration machine. To research the vibration machines strongly nonlinear and high vibration intensity characteristic such as certain excitation and uncertain response, the vibration machine with its double-mass is built, and its vibration exciter uses two partial blocks as vibration motor. In addition, dynamic vibration differential equation is established. To achieve high vibration intensity results based on the vibration machines safe working, the advanced control based on the SCM and Intelligent frequency conversion is put forward, and the advanced control system with its host computer, frequency converter, SCM, charge-amplifier, sensor and the vibration machine is been established.

INTRODUCTIONHerpes simplex virus type 2 (HSV-2) is the most common cause of genital herpes. Glycoprotein G (gG) is a prototype antigen for type-specific serodiagnosis distinguishing between HSV type 1 (HSV-1) and HSV-2 infections. As immunological diagnosis kits for accurate differentiation between HSV-1 and HSV-2 antibodies can be expensive, there is a need to develop a convenient, sensitive, specific and cost-effective serodiagnostic kit. METHODSWe successfully expressed a fragment of gG comprising residues 321-580 of HSV-2 with histidine tag (gG 321-580His ) in a Bac-to-Bac baculovirus expression system, which had an antigenicity similar to its native counterpart. An indirect enzyme-linked immunosorbent assay (ELISA) was developed using gG 321-580His as the diagnostic antigen and evaluated by comparison with a commercial HerpeSelect 2 ELISA immunoglobulin G kit as reference. RESULTSIn testing 318 field serum samples, the diagnostic relative sensitivity and specificity of the developed gG 321-580His -ELISA test in qualitative comparison with the commercial kit were 93.81% and 96.74%, respectively, and the accuracy was 94.65%. CONCLUSIONThe study indicates that gG 321-580His has a high diagnostic potential for HSV-2 virus serodiagnosis in humans. Keywords: baculovirus expression system, glycoprotein G, herpes simplex virus type 2, immunodominant fragment, indirect enzyme-linked immunosorbent assay

A pushrod harmonic institution which is consisted of harmonic planetary and cam mechanism is proposed. As a result, speed-down device of low-speed motor is changed from external installed to interior installed. Design goals of the transmission with small size, light weight and low cost is achieved. Based on the mechanism principle analysis, the theoretical profile equation of the pushrod roller is deduced, then the displacement, velocity and acceleration equation of a random points are got by the analysis method. It is helpful to analyze the change of each motion parameters and the amplitude for the influence of the movement characteristics, at the same time provides evidence for mechanism simulation, kinematics parameters optimization and high-quality manufacturing of the equipment.

The effective control of high-vibration intensity frequency and its overtime and over-limited is a bottleneck technology of chaotic vibration machine to finish specific operation. A PLC advance control system based on intelligent frequency is developed. Prediction of the possible vibration intensity extreme points and its distribution are processed data mining and prediction by sensor signal amplification, analog-digital conversion, and online monitor. Moreover, advanced control of the overtime and over-limited vibration intensity is achieved by constraint conditions correction and real-time feedback. The advanced control results can be verified by comparing grinding effect of conventional vibration mill with that of advanced control vibration mill. Further, the deaggregation and refinement of super-hard and superfine grinding can be achieved.

Some special operations of chaotic vibration mill require not only has a certain frequency high vibration intensity but also can effectively control overtime and over-limited of vibration intensity. The validity of advanced control decision is key technology for chaotic vibration mill to achieve some special operations such as super-hard and superfine grinding. Through the application based on the intelligent frequency conversion of PLC advanced control system, the high vibration intensity exceeds 15 presents discontinuously and the transient super-high vibration intensity exceeds 20 presents 3 times shortly. By comparing with conventional vibration mill, it shows that the advance control system can control high vibration intensity effectively, and the engineering effects of the deaggregation and refinement of super-hard and superfine grinding is obtained.

The key technology of vibration machine to finish deaggregation and powder refinement is both high vibration Intensity with a certain frequency and steady vibration of frequency conversion control. A frequency conversion control for vibration mill system based on multi-wave variable sinusoidal frequency ascending and descending cycle curve was developed. The frequency conversion control test of vibration mill was finished through sensing signal amplification, analog digital conversion and on line detection. The results show that the high and super-high vibration intensity with certain action time is produced and the effect of multi-amplitude and multi-frequency is acquired. The experimental data show that the super micro grinding of superfine particles is achieved, energy consumption is reduced, the useful life of wearing parts such as bearing is extended, and engineering effects of deaggregation and refinement of super-hard superfine powder are obtained by frequency conversion control.