Griffonia simplicifolia lectin II (GSII) and wheat germ agglutinin (WGA) are N-acetylglucosamine-binding lectins. Previous studies demonstrated that they have anti-insect activity, a property potentially useful in pest control. To gain some insight into the insect response to dietary lectins, we performed transcriptomic analysis using the cowpea bruchid (Callosobruchus maculatus) midgut microarray platform we built. Compared to the nonnutritional cellulose treatment, dietary lectins induced more profound changes in gene expression. Ingestion of relatively high doses of lectins for 24 h resulted in alteration of gene expression involved in sugar and lipid metabolism, transport, development, defense, and stress tolerance. Metabolic genes were largely downregulated. Moreover, we observed disorganized microvilli resulting from ingestion of WGA. This morphological change is consistent with the lectin-induced changes in genes related to midgut epithelial cell repair. In addition, suboptimal nutrient conditions may serve as a stress signal to trigger senescence processes, leading to growth arrest and developmental delay.
In this paper, the law of hydration expansion of polymer microsphere and the plugging rate of polymer microsphere under condition of different influencing factors such as hydration time, mass concentration, and permeability are studied. The applicable conditions are optimized as following: The optimum hydration time, mass concentration, and formation permeability through laboratory experiment is 5 days 3000 mg/L~4000 mg/L, permeability under 4 um2respectively. Finally, oil displacement experiment is carried out under laboratory conditions. The results show that the enhancement in oil recovery obtained in core flow experiment is about 7 %, which suggests that it has a wide application prospects in tertiary oil recovery.
In order to further enhance oil recovery (EOR) of the high temperature and high salt oil fields by foam flooding, one foaming agent was screened by airflow method. The influence of oil-water and pressure on foamability and stability were evaluated,and oil displacement experiment was also conducted. The results show that CS-1 foaming agent has better foamability and stability than the others under the reservoir conditions (Temperature 110 °C, Salinity 11.52×104 mg/L, Ca2+&Mg2+ 7654 mg/L). The foam stability will be better as the pressure rise or be worse when it met the crude oil. Oil recovery was improved by 4.13% after waterflood and the total recovery is 60.75%.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.