Details of elastin’s complex
conformational and dynamic
heterogeneity were acquired from one- and two-dimensional solid-state
nuclear magnetic resonance (ssNMR) experiments on hydrated elastin
that is isotopically enriched at its alanines. Elastin’s abundant
alanines are useful probes of the structural and dynamical microenvironments
in its cross-linking and hydrophobic domains. High isotopic enrichment
of alanines in neonatal rat smooth muscle cells (NRSMC) elastin was
obtained with the inhibition of alanine transaminase, combined with
an excess of [U–13C]alanine in the culture media.
Due to the fast, large-amplitude motions, R-TOBSY was utilized with
selective homonuclear decoupling schemes to resolve 13C-Ala
peaks and confirm assignments. A data-driven approach is applied,
as the interpretation of chemical shifts is based on the distribution
of conformation-dependent 13C-Ala chemical shifts in proteins
in multiple databases. Alanine populations in elastin’s hydrophobic
domains are primarily random coil, whereas those in its cross-linking
regions reside in α-helices and random coils.
Elastin is the insoluble elastomeric protein that provides extensibility and resilience to vertebrate tissues. Limited high-resolution structural data for elastin are notably complex. To access this information, this protein is considered in the simplified context of its two general domain types, that is, hydrophobic (HP) and crosslinking (CL). The question of elastin's structure−function has directed the focus of nearly all previous studies in the literature to the unique repeating sequences characteristic of this protein, found primarily in the HP domains. The CL domains were assumed to play a very limited role in biological elasticity due in part to the significant α-helical character that was (incorrectly) predicted for these regions. In this study, the conformational heterogeneity of alanines in native elastin's CL domains is examined in the context of helix−coil transition theory (HCTT) using solid-state nuclear magnetic resonance (SSNMR) spectroscopy in tandem with strategic isotopic labeling. Helix and coil populations are observed at all temperatures, but the former increases significantly at lower temperatures. Below the glass transition temperature (T g ), two major populations of alanines in the CL regions are resolved by two-dimensional SSNMR; one-dimensional methods are used for characterization in nativelike conditions. The spectra of 13 CO-Ala in the CL regions are simulated using an HCTT-based statistical mechanical representation. Below T g , longer segments with significant helical probabilities are consistent with the experimental data. At higher temperatures, the SSNMR lineshapes are best fit with a distribution of shorter (Ala) n segments, most in random coil. These results are used to refine a structure−function model for elastin in the context of HCTT, redirecting attention to the CL domains and their role in elasticity.
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