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Plant proteases are capable of performing several functions in biological systems, and their use is attractive for biotechnological process due to their interesting catalytic properties. Bromelia pinguin (aguama) is a wild abundant natural resource in several regions of Central America and the Caribbean Islands but is underutilized. Their fruits are rich in proteases with properties that are still unknown, but they represent an attractive source of enzymes for biotechnological applications. Thus, the proteolytic activity in enzymatic crude extracts (CEs) from wild B. pinguin fruits was partially characterized. Enzymes in CEs showed high proteolytic activity at acid (pH 2.0-4.0) and neutral alkaline (pH 7.0-9.0) conditions, indicating that different types of active proteases are present. Proteolytic activity inhibition by the use of specific protease inhibitors indicated that aspartic, cysteine, and serine proteases are the main types of proteases present in CEs. Activity at pH 3.0 was stable in a broad range of temperatures (25-50 °C) and retained its activity in the presence of surfactants (SDS, Tween-80), reducing agents (DTT, 2-mercapoethanol), and organic solvents (methanol, ethanol, acetone, 2-propanol), which suggests that B. pinguin proteases are potential candidates for their application in brewing, detergent, and pharmaceutical industries.

Nowadays agriculture is facing constantly challenges to produce more and better food for a growing population under a changing climate scenery. To afford this demand, agricultural practices must innovate in new methods for crop production, nutrition management, and plant health. In addition, the development of new compounds or biological agents able to improve plant yield (regulating plant physiology, metabolism, crop performance), and agro-product quality, are also necessary. In the last decade, agents with these characteristics have been proposed for a sustainable agriculture. These agents, defined as biostimulants, have showed to improve plant nutrition, quality, yield and abiotic tolerance in different agricultural crops. In particular, the use of protein hydrolysates as biostimulants offers promising results to reduce the use of agrochemicals and improve productivity parameters in a variety of cultivars, in agree with the modern agricultural production challenges. The present review is focused in the use of protein hydrolysates as plant biostimulants, describing typical and potential agro industrial by-products as sources substrates for protein-based biostimulants formulation, and commercial or experimental proteolytic enzymes used for hydrolysate production. Moreover, critical aspects during protein biostimulants manufacture, functional profiling and its applications are discussed. Finally, recent advances for the evaluation of protein hydrolysates on plant physiology and metabolism under experimental and commercial scale are also documented. Concluding in the necessity to conduct structural studies, in order to identify and produce bioactive peptides with agricultural applications.

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