This paper reports a comparison of the antibacterial properties of copper-amino acids chelates and copper nanoparticles against Escherichia coli, Staphylococcus aureus, and Enterococcus faecalis. These copper-amino acids chelates were synthesized by using a soybean aqueous extract and copper nanoparticles were produced using as a starting material the copper-amino acids chelates species. The antibacterial activity of the samples was evaluated by using the standard microdilution method (CLSI M100-S25 January 2015). In the antibacterial activity assays copper ions and copper-EDTA chelates were included as references, so that copper-amino acids chelates can be particularly suitable for acting as an antibacterial agent, so they are excellent candidates for specific applications. Additionally, to confirm the antimicrobial mechanism on bacterial cells, MTT assay (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) was carried out. A significant enhanced antimicrobial activity and a specific strain were found for copper chelates over E. faecalis. Its results would eventually lead to better utilization of copper-amino acids chelate for specific application where copper nanoparticles can be not used.
The preparation of stable, uniformed silver nanoparticles by the reduction of silver ions using albumin is reported in the present paper. It is a simple process for obtaining silver nanoparticles. The samples have been characterized by X-ray diffraction (XRD) and transmission electron microscopy (TEM), which reveal the nature of the nanoparticles. These studies conclude that the particles are mostly spherical in shape and have an average size of 26 nm. The UV-vis spectra show that an absorption band, occurred because of the Surface Plasmon Resonance, existing at 443 nm; the Thermogravimetric Analysis (TGA) shows that only 2% of the weight is organic matter. The average particle size was measured with a Nanosizer DLS
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