The biochemical methane potential test is the most commonly applied method to determine methane production from organic wastes. One of the parameters measured is the volume of biogas produced which can be determined manometrically by keeping the volume constant and measuring increases in pressure. In the present study, the effect of pressure accumulation in the headspace of the reactors has been studied. Triplicate batch trials employing cocoa shell, waste coffee grounds and dairy manure as substrates have been performed under two headspace pressure conditions. The results obtained in the study showed that headspace overpressures higher than 600mbar affected methane production for waste coffee grounds. On the contrary, headspace overpressures within a range of 600-1000mbar did not affect methane production for cocoa shell and dairy manure. With the analyses performed in the present work it has not been possible to determine the reasons for the lower methane yield value obtained for the waste coffee grounds under high headspace pressures.
Three different types of anaerobic sludge (granular, thickened digestate and anaerobic sewage) were evaluated as seed inoculum sources for the high rate anaerobic digestion of pig slurry in UASB reactors. Granular sludge performance was optimal, allowing a high efficiency process yielding a volumetric methane production rate of 4.1LCHLd at 1.5days HRT (0.248LCHgCOD) at an organic loading rate of 16.4gCODLd. The thickened digestate sludge experimented flotation problems, thus resulting inappropriate for the UASB process. The anaerobic sewage sludge reactor experimented biomass wash-out, but allowed high process efficiency operation at 3days HRT, yielding a volumetric methane production rate of 1.7LCHLd (0.236LCHgCOD) at an organic loading rate of 7.2gCODLd. To guarantee the success of the UASB process, the settleable solids of the slurry must be previously removed.
This study is the first test that proves high rate anaerobic digestion as an efficient technological process for the treatment of gin spent wash. The gin spent wash was codigested in UASB reactors with swine wastewater, which provided nutrients and alkalinity. The process was optimized by increasing the proportion of gin spent wash in the feed, and thus the organic loading rate (OLR) up to reactor failure. Stable highefficiency operation was reached at an OLR as high as 28.5 kg COD m -3 d -1 , yielding 8.4 m 3 CH4 m -3 d -1 and attaining a COD removal of 97.0%. At an organic loading rate of 32.0 kg COD m -3 d -1 , the process became unstable and the reactor underwent overacidification that drastically lowered the pH and suppressed methanogenesis. The failure of the reactor was caused by a combination of an organic overloading and alkalinity deficit that uncoupled acidogenesis and methanogenesis.
The impact of a conductive material as powdered activated carbon (PAC) on the biochemical methane potential of whisky pot ale (PA) and brewery spent yeast (SY) was investigated. The test was carried out with three different types of anaerobic inocula: manure inoculum (MI), sewage sludge (SS) and granular sludge (GR). Brewery spent yeast produced partial (in sewage and granular sludge) and total (in manure inoculum) methanogenesis inhibition due to the toxicity of some of its constituents (hops extract). The inhibition was overcome by the supplementation of PAC, that improved significantly the anaerobic digestion process for spent yeast, allowing to reach biochemical methane potential values between 657-699 L CH4 kg-1 VS and it reduced redox potential from −368 mV to −398 mV. The activated carbon did not improve the methane yields from whisky pot ale since microorganisms did not have difficulties to process this substrate; in fact, the redox potential slightly increased from-355 mV to-330 mV.
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