Handroanthus impetiginosus is a tree species with ecological and economic potential. Despite that, in the Brazilian market, its wood is heavily exploited in the illegal trade. Therefore, studies on genetic diversity are necessary in order to propose strategies for conservation of this species. Thus, the aim of this study was to select Inter Simple Sequence Repeat (ISSR) primers for genetic diversity studies applied to the forest population of H. impetiginosus. For this, 30 ISSR molecular primers were tested in 30 individuals, evaluating the total number of loci, polymorphism rate and polymorphic information content, as well as marker index and resolving power. Eight primers were selected for having a better amplification pattern, which provided 62 loci. The polymorphic information content of the primers ranged from 0.34 to 0.49, while the marker index (MI) averaged 3.20, with resolving power (RP) of 2.40, Nei’s diversity (He) of 0.35 and Shannon index (I) of 0.52. The results show that the primers UBC 807, 809, 818, 824, 857, 860, 873 and 881 are efficient for quantifying the genetic diversity of H. impetiginosus. These results can contribute to supporting strategies aimed at the conservation of this species and selection of parent trees.
Cordia oncocalyx Allemão Baill., widely known as “pau-branco”, is a native species from the Caatinga (Brazilian Savannah) and has socioeconomic and environmental potential; however, there are few silvicultural studies on this species. Therefore, this paper aimed to analyze and compare the biometric characteristics of diaspores and seeds through manual biometrics and by digital image processing, and to evaluate the in vitro and ex vitro germination of C. oncocalyx. In the biometrics evaluation, three hundred diaspores and three hundred seeds were used, applying manual and digital biometrics. Subsequently, ex vitro emergence was determined, testing mechanical scarification (in different regions of the diaspore) and chemical scarification (immersion in sulfuric acid for 90 min and 180 min). Finally, in vitro germination was tested with different compositions of Murashige & Skoog (M&S) culture medium and sucrose addition. Results showed that digital image processing is a viable and fast technique to obtain the biometric parameters of C. oncocalyx fruit and seeds. Chemical and mechanical treatments on diaspores have not influenced seed emergence (0.33%). The composition of the culture medium has influenced the germination percentage, and the maximum value of 96% % was obtained with 6 g/L of sucrose and 0.90 g/L of M&S medium. Thus, the seminiferous propagation of C. oncocalyx can be performed successfully when the seeds are germinated in vitro, and the digital image processing shows the solidity and applicability aiming to evaluate the quantitative parameters of seed and fruit of this species.
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