Pregnancy in mammals requires remodeling of the uterus to become receptive to the implanting embryo. Remarkably similar morphological changes to the uterine epithelium occur in both eutherian and marsupial mammals, irrespective of placental type. Nevertheless, molecular differences in uterine remodeling indicate that the marsupial uterus employs maternal defences, including molecular reinforcement of the uterine epithelium, to regulate embryonic invasion. Non-invasive (epitheliochorial) embryonic attachment in marsupials likely evolved secondarily from invasive attachment, so uterine defences in these species may prevent embryonic invasion. We tested this hypothesis by identifying localization patterns of Talin, a key basal anchoring molecule, in the uterine epithelium during pregnancy in the tammar wallaby (Macropus eugenii; Macropodidae) and the brush tail possum (Trichosurus vulpecula; Phalangeridae). Embryonic attachment is non-invasive in both species, yet Talin undergoes a clear distributional change during pregnancy in M. eugenii, including recruitment to the base of the uterine epithelium just before attachment, that closely resembles that of invasive implantation in the marsupial species Sminthopsis crassicaudata. Basal localization occurs throughout pregnancy in T. vulpecula, although, as for M. eugenii, this pattern is most specific prior to attachment. Such molecular reinforcement of the uterine epithelium for non-invasive embryonic attachment in marsupials supports the hypothesis that less-invasive and non-invasive embryonic attachment in marsupials may have evolved via accrual of maternal defences. Recruitment of basal molecules, including Talin, to the uterine epithelium may have played a key role in this transition.

Land clearing for agriculture results in fragmented landscapes with diminished resources for wildlife. Habitat loss and fragmentation are widespread problems; however, their impacts are rarely assessed simultaneously at multiple spatial and temporal scales. We used a focal site, multiscale design to examine how landscape characteristics that affect the probability of koala Phascolarctos cinereus presence varies over multiple spatial and temporal scales in a fragmented rural landscape in north‐western New South Wales, Australia. We modelled the effect of variables representing landscape characteristics at three spatial extents – transect, patch and site – over three seasonal periods (mid‐spring, late‐spring and mid‐summer). Koala activity level (as determined by faecal pellets) varied between surveys, and identifies short‐term seasonal changes in habitat use. Koala presence had the strongest association with patch scale variables (habitat suitability, soil fertility and patch area). Temporal variation in the effect of tree and landscape characteristics on koala presence highlights the limitations of surveys at a single point in time, and emphasizes that further research is needed to advance our understanding of what drives temporal variation in koala habitat use at multiple spatial scales. Our results show that to arrest declining koala populations in rural agricultural landscapes, conservation and management plans must consider landscape scale processes over a variety of time scales, and engage stakeholders across multiple properties and tenures, to conserve both disturbed and suitable habitat on private land.

Context Precise and accurate estimates of animal numbers are often essential for population and epidemiological models, as well as for guidance for population management and conservation. This is particularly true for threatened species in landscapes facing multiple threats. Estimates can be derived by different methods, but the question remains as to whether these estimates are comparable. Aims We compared three methods to estimate population numbers, namely, distance sampling, mark–recapture analysis, and home-range overlap analysis, for a population of the iconic threatened species, the koala (Phascolarctos cinereus). This population occupies a heavily fragmented forest and woodland habitat on the Liverpool Plains, north-western New South Wales, Australia, on a mosaic of agricultural and mining lands. Key resultsAll three methods produced similar estimates, with overlapping confidence intervals. Distance sampling required less expertise and time and had less impact on animals, but also had less precision; however, future estimates using the method could be improved by increasing both the number and expertise of the observers. ConclusionsWhen less intrusive methods are preferred, or fewer specialised practitioners are available, we recommend distance sampling to obtain reliable estimates of koala numbers. Although its precision is lower with a low number of sightings, it does produce estimates of numbers similar to those from the other methods. However, combining multiple methods can be useful when other material (genetic, health and demographic) is also needed, or when decisions based on estimates are for high-profile threatened species requiring greater confidence. We recommend that all estimates of population numbers, and their precision or variation, be recorded and reported so that future studies can use them as prior information, increasing the precision of future surveys through Bayesian analyses.