Article history Foodborne diseases represent a global public health issue and dairy products are closely related to it, since the quality of the milk produced in several Brazilian regions is unsatisfactory due to the presence of microorganisms in it. Listeria monocytogenes is a serious problem linked to food safety and, when it comes to milk, it represents a potential danger because it can withstand food storage temperatures, among other characteristics. The significant participation of Alagoas State in this the dairy production sector and the importance of producing safe food led to the aim of the present study, namely: investigating Listeria monocytogenes in expansion tank milk in Alagoas State counties. Milk samples were collected from tanks in 30 milk-processing unit suppliers. Next, they were taken to the Meat and Milk Inspection Laboratory (LICAL-UFRPE). The ISO 11290-1: 1996 / Amd.1: 2004 method, with adaptations, was used in the analyses. Bluish colonies, with or without halo formation, were identified according to their morpho-tintorial and biochemical characteristics. Listeria monocytogenes were detected in 20% of the samples (6/30), and such rate represents a public health risk. Thus, monitoring this microorganism and mastitis in the herd, as well as pre-and post-dipping, equipment and storage tank sanitation, and milk collection through refrigerated trucks at the appropriate time is a relevant procedure to prevent contamination and to assure safe food provision to the population.
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The aim of this study was to investigate Listeria monocytogenes in ham sliced in supermarkets in Recife city, Pernambuco state. In total, 40 samples of sliced ham were collected, and 25 g of ham was added to 225 mL of Demi Fraser broth. After incubation, 0.1 mL was inoculated in Fraser broth and, subsequently, sown in supplemented Listeria Selective Agar, based on Otaviani and Agosti. The following tests were carried out for confirmation purposes: Gram stain, motility test, catalase test and cAMP test. There was L. monocytogenes in 25% (10/40) of the samples. The presence of L. monocytogenes in ready-to-eat food, such as sliced ham, is likely related to lack of proper equipment-cleaning in supermarkets, a fact that poses great risk to public health.
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