Biobank saliva sample quality depends on specific criteria applied to collection, processing, and storage. In spite of the growing interest in saliva as a diagnostic fluid, few biobanks currently store large collections of such samples. The development of a standard operating procedure (SOP) for saliva collection and quality control is fundamental for the establishment of a new saliva biobank, which stores samples to be made available to the saliva research community. Different collection methods were tested regarding total volume of protein obtained, protein content, and protein profiles, and the results were used to choose the best method for protein studies. Furthermore, the impact of the circadian variability and inter- and intraindividual differences, as well as the saliva sample stability at room temperature, were also evaluated. Considering our results, a sublingual cotton roll method for saliva collection proved to produce saliva with the best characteristics and should be applied in the morning, whenever possible. In addition, there is more variability in salivary proteins between individuals than in the same individual for a 5-month period. According to the electrophoretic protein profile, protein stability is guaranteed for 24 hours at room temperature and the protein degradation profile and protein identification were characterized. All this information was used to establish an SOP for saliva collection, processing, and storage in a biobank. We conclude that it is possible to collect saliva using an easy and inexpensive protocol, resulting in saliva samples for protein analysis with sufficient quality for biobanking purposes.
O catalisador nanoestruturado SiO 2 /ZrO 2 foi preparado pelo método sol-gel e apresentou uma área superficial de 131,50 ± 14 m 2 g-1. As imagens de microscopia eletrônica de varredura mostraram que o catalisador apresentou partículas muito homogêneas de 250 nm de diâmetro. Este material foi aplicado na transesterificação do óleo de soja para a produção de biodiesel. A conversão do óleo em biodiesel teve um rendimento de 96,2 ± 1,4 % em 3 h de reação de transesterificação catalisada pelo material SiO 2 /ZrO 2 (SiZr). O catalisador foi recuperado e reutilizado por seis vezes, mantendo uma eficiência catalítica de 84,1%. Nanosized SiO 2 /ZrO 2 catalyst with high surface area (131.50 ± 14 m 2 g-1) was prepared by the sol-gel method. The material presented highly homogeneous particles with an average diameter of 250 nm according to SEM images. It was applied in the transesterification of soybean oil for biodiesel production. The biodiesel conversion reached a yield of 96.2 ± 1.4 % after 3 h of transesterification reaction catalised by SiO 2 /ZrO 2 (SiZr). The catalyst was recovered and reused six times, maintaining a catalytic efficiency of about 84.1 %.
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