The success of topically applied treatments on skin relies on the efficacy of skin penetration. In order to increase particle or product penetration, mild skin barrier disruption methods can be used. We previously described cyanoacrylate skin surface stripping as an efficient method to open hair follicles, enhance particle penetration, and activate Langerhans cells. We conducted ex vivo and in vivo measurements on human skin to characterize the biological effect and quantify barrier disruption-related inflammation on a molecular level. Despite the known immunostimulatory effects, this barrier disruption and hair follicle opening method was well accepted and did not result in lasting changes of skin physiological parameters, cytokine production, or clinical side effects. Only in ex vivo human skin did we find a discrete increase in IP-10, TGF-β, IL-8, and GM-CSF mRNA. The data underline the safety profile of this method and demonstrate that the procedure per se does not cause substantial inflammation or skin damage, which is also of interest when applied to non-invasive sampling of biomarkers in clinical trials.
We propose, for the first time, that IL-32 is a molecular link between KCs and LCs in healthy skin, provoking LC migration from the epidermis to the dermis prior to their migration to the draining lymph nodes.
Objectives: Psoriasis-related pruritus is a frequent and underestimated symptom with a strong impact on quality of life, psychological condition and sleep. Our main objective was to document, in real-life clinical practice, the perception of pruritus and the efficacy of a 4-week topical treatment with a fixed-dose combination of calcipotriol/betamethasone dipropionate (Cal/BD) foam on psoriasis-related moderate-to-severe pruritus. Methods: This was an exploratory prospective observational study in psoriasis patients with a pruritus visual analogic scale (VAS) score ≥ 3/10 cm. The primary endpoint was the proportion of responder patients defined as the selfassessed improvement from baseline of pruritus of at least 2 VAS score points 48 h after the first topical administration. The quality of sleep was assessed on VAS and the quality of life was assessed using the Dermatology Life Quality Index (DLQI) on Day 28.Results: A total of 81 patients were included by 34 dermatologists and 40 selfquestionnaires were evaluable for efficacy on Day 28. At inclusion, pruritus was self-assessed as severe by 60.8% of patients, whereas physicians reported severe pruritus in 16.9% of patients. A proportion of 91.4% (95% confidence interval: 82-100) of patients were responders to pruritus relief 48 h after the first Cal/BD application. Relevant improvements in quality of sleep after 24 h (+2.9 ± 3.0) and in quality of life after 28 days (−9.0 ± 6.8) were reported.
Conclusion:This exploratory study provides further evidence that pruritus is an underestimated symptom, and it confirms the usefulness of the fixed-dose combination of Cal/DB foam in relieving moderate-to-severe pruritus and improving the quality of life and sleep in patients with plaque psoriasis.
trifluoro-1-isopropyl-2-oxopropyl]aminocarbonyl] pyrrolidin-1-yl] carbonyl]-2-methylpropyl]aminocarbonyl]benzoylamino]acetate (NEI-L1) was approved as a quasi-drug ingredient to improve wrinkles in 2016 in Japan. The product containing NEI-L1 has been used by many Japanese women in the past two years. NEI-L1 has the strong effect to inhibit the activity of neutrophil elastase (NE). Neutrophils are migrated into dermis by stimuli such as ultraviolet irradiation and they release NE to degrade dermal extracellular matrix (ECM). NEI-L1 suppresses degradation of ECM and improves the wrinkle. In this study, we examined whether NEI-L1 suppresses NE-induced collagen degradation using the culture supernatant of normal human dermal fibroblasts in vitro experiment and cultured human skin tissues ex vivo experiment. As a result of in vitro experiment, collagen content in supernatant treated with 2.5 mg/mL NE was significantly reduced (4.6%) compared to non-treated control. On the other hand, 10 mg/mL NEI-L1 drastically suppressed this
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.