An automated method has been developed for determining serum iron-binding capacity and urinary iron as well as serum iron. After preliminary preparation, the sample is treated with ascorbic acid in hydrochloric acid to release and reduce protein-bound iron, then singly dialyzed into an acetate buffer and reacted with 4,7-diphenyl-1,10-phenanthroline (bathophenanthroline) to form a pink color proportionate to the amount of iron present. The sample passes through a 15-mm flow cuvet and is read at 537.5 nm, with the range expander set at either 2x or 4x.

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