Temperature increases associated with global climate change are likely to be accompanied by additional environmental stressors such as desiccation and food limitation, which may alter how temperature impacts organismal performance. To investigate how interactions between stressors influence thermal tolerance in the common forest ant, Aphaenogaster picea, we compared the thermal resistance of workers to heat shock with and without pre-exposure to desiccation or starvation stress. Knockdown (KD) time at 40.5 °C of desiccated ants was reduced 6% compared to controls, although longer exposure to desiccation did not further reduce thermal tolerance. Starvation, in contrast, had an increasingly severe effect on thermal tolerance: at 21 days, average KD time of starved ants was reduced by 65% compared to controls. To test whether reduction in thermal tolerance results from impairment of the heat-shock response, we measured basal gene expression and transcriptional induction of two heat-shock proteins (hsp70 and hsp40) in treated and control ants. We found no evidence that either stressor impaired the Hsp response: both desiccation and starvation slightly increased basal Hsp expression under severe stress conditions and did not affect the magnitude of induction under heat shock. These results suggest that the co-occurrence of multiple environmental stressors predicted by climate change models may make populations more vulnerable to future warming than is suggested by the results of single-factor heating experiments.
The Anatolian Peninsula, comprising most of modern Turkey, is situated at the junction of Europe, the Middle East and Asia. Together with its complex geomorphological and climatic history, this has given rise to a rich fauna and flora, which exhibits a wide range of historical biogeographical patterns. The stone marten (Martes foina) is a small carnivore that is found across the temperate Palaearctic region including Anatolia, where it is often associated with habitats modified by humans, but few genetic data exist for this species. We sequenced a 1840-bp region of the mitochondrial genome from 97 martens sampled across the peninsula and intron 7 of the nuclear β-fibrinogen gene from 53 of these. Two mitochondrial lineages were recovered, with overlapping eastern and western distributions, but there was no geographical structure for the autosomal marker. Coalescent analyses indicated that both of the lineages originated during the Last Glacial Maximum, one of them within an eastern Anatolian refugium and the other in a western Anatolian or Balkan refugium. The western lineage colonized most of Europe in the Holocene, while the eastern lineage may be endemic to Anatolia, from where it colonized the Iberian Peninsula via human translocation. The presence of at least one refugial stone marten population highlights the importance of Anatolia to the preservation of genetic variation and biodiversity.
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