Aim: The prevalence, sensitivity profile and resistance of Gram-positive bacteria in wounds to commercial antibiotics were ascertained in this study. Place and duration of study: University of Medical Sciences Teaching Hospital, Akure, Nigeria, between January and June 2019. Methodology: Wound swabs sample collection, isolation of bacteria, identification of Gram-positive bacteria isolates and antibiotics sensitivity testing of isolated bacteria were determined employing standard protocols. Result: Three Gram-positive bacteria were isolated and presumptively identified to be S. aureus, S. epidermidis and S. pyogenes. S. aureus had the highest prevalence of 53% followed by S. epidermidis with 42% and S. pyogenes accounting for the least occurrence of 5%. Ninety percent (90%) of ten S. aureus strains were resistant to ciprofloxacin while only 10% had intermediate activity. The least resistance of S. aureus strains was against pefloxacin (40%), while to streptomycin, 87.5% of eight S. epidermidis strains were resistant and 12.5% had intermediate sensitivity. Susceptibility was observed in S. epidermidis against pefloxacin (12.5%) while 50% had intermediate sensitivity and 37.5% were resistant. The highest zone of inhibition of S. epidermidis was observed in strain 7 against pefloxacin (16.00±1.00 mm) and in S. aureus by strain 5 against pefloxacin (16.50±2.50 mm). Conclusion: Pefloxacin-sensitive Staphylococcus and Streptococcus species from wound swabs could become resistant overtime and this calls for incessant vigilance on Gram-positive wound bacteria antibiotic-susceptibility appraisal particularly in an antibiotics-abuse setting.
Aim: To investigate the antimicrobial susceptibility profile of Pseudomonas aeruginosa enumerated from wound swabs and urine samples from the University of Medical Sciences Teaching Hospital, Akure, Nigeria. Place of Study: University of Medical Sciences Teaching Hospital, Akure, Ondo State, Nigeria, between January and May, 2019. Methodology: Wound swabs and urine samples were collected from patients of University of Medical Sciences Teaching Hospital, Akure. Enumeration and identification of P. aeruginosa isolates was employed. Antibiotic sensitivity test was conducted on the enumerated P. aeruginosa strains from both clinical specimens via standard disc diffusion protocol. The susceptibility and resistance pattern of P. aeruginosa isolates was established utilizing clinical laboratory standard institute (CLSI) standard. Results: Ciprofloxacin was observed to display the highest zone of inhibition (ZOI) of 17.00±1.00 mm for P. aeruginosa isolate 1 and likewise the highest ZOI of 24.50±1.50 mm for P. aeruginosa strain 3. Fourteen strains of Pseudomonas aeruginosa exhibited highest resistance to septrin and augmentin for wound swabs as all 19 of the bacterial strains also exhibited the highest resistance to septrin, chloramphenicol, augmentin and streptomycin for urine specimens. Ten (10) of P. aeruginosa strains from wound swabs exhibited the highest intermediate susceptibility to perfloxacin. Eleven (11) strains of P. aeruginosa from urine specimens exhibited the highest intermediate susceptibility on sparfloxacin. All 19 strains of P. aeruginosa from urine specimens were susceptible to amoxicillin and gentamicin as completely minimal susceptibility was recorded for P. aeruginosa associated with wound swabs. Conclusion: This study demonstrated the high resistance pattern of P. aeruginosa associated with wound swabs and urine samples and emphasizes the need for the regimentation of over-the-counter remedy and antibiotic susceptibility appraisal of anti-pseudomonal drugs.
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