Abstract. Gear systems are extensively employed in mechanical systems since they allow the transfer of power with a variety of gear ratios. So gears cause the inherent deflections and deformations due to the high pressure which occurs between the meshing teeth when transmit power and results in the transmission error. It is usually assumed that the transmission error and variation of the gear mesh stiffness are the dominant excitation mechanisms. Predicting the static transmission error is a necessary condition to reduce noise radiated from the gear systems. This paper aims to investigate the effect of tooth profile modifications on the transmission error of helical gear. The contact stress analysis was implemented for different roll positions to find out the most critical roll angle in view point of root flank stress. The PPTE (peak-to-peak of transmission error) is estimated at the roll angles by different loading conditions with two dimensional FEM. The optimal profile modification from the root to the tip is proposed.
Background: Delayed wound healings in diabetic patients are related with the impairment of the expressions of various growth factors. Treatments using growth factors have been attempted on diabetic foot ulcer. VEGF (vascular endothelial growth factor) accelerates neo-angiogenesis on the early phase of the wound healing and exerts chemo-attractive effect for the other growth factors and cytokines. Non-viral gene transfer strategies are attractive tool for the gene therapy due to the safety and the versatility, but the low efficiency has been the serious problem. Methods: We performed the VEGF gene therapy using reconstructed minicircle MINI-pβVEGF DNA with a polymeric carrier, polyethylenimine (PEI, 25 kDa) in HEK293, CHO, and NIH3T3 cell lines, and compared its efficiency with the conventional VEGF plasmid pβVEGF.
Results:The levels of expressed VEGF were higher in the groups using BPEI (branched PEI) as a gene carrier than naked plasmid transfer in all cell lines (P < 0.05). The minicircle MINI-pβVEGF DNA showed much higher VEGF expression than conventional plasmid pβVEGF (P < 0.05).
Conclusion:Minicircle DNA MINI-pβVEGF showed much higher transfection efficiency than conventional plasmid pβVEGF. It might be used in actual human clinical trial due to its higher efficiency and possible safety for the treatment of diabetic foot ulcer.
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