Lactobacillus reuteri AN417 is a newly characterized probiotic strain. The activity of AN417 against oral pathogenic bacteria is unknown. We investigated the antibacterial activity of cell-free L. reuteri AN417 culture supernatant (LRS) against three oral pathogens: Porphyromonas gingivalis, Fusobacterium nucleatum, and Streptococcus mutans. P. gingivalis and F. nucleatum have been implicated in periodontal disease, whereas S. mutans causes dental caries. Exposing these oral pathogenic bacteria to LRS significantly reduced their growth rates, intracellular ATP levels, cell viability, and time-to-kill. The minimal inhibitory volume of LRS was 10% (v/v) against P. gingivalis, 20% (v/v) for F. nucleatum, and 30% (v/v) for S. mutans. LRS significantly reduced the integrity of biofilms and significantly suppressed the expression of various genes involved in P. gingivalis biofilm formation. The L. reuteri AN417 genome lacked genes encoding reuterin, reuteran, and reutericyclin, which are major antibacterial compounds produced in L. reuteri strains. LRS treated with lipase and α-amylase displayed decreased antibacterial activity against oral pathogens. These data suggest that the antibacterial substances in LRS are carbohydrates and/or fatty acid metabolites. Our results demonstrate that LRS has antimicrobial activity against dental pathogenic bacteria, highlighting its potential utility for the prevention and treatment of P. gingivalis periodontal disease.

The purpose of this study is to determine whether the lower intakes of yogurt, milk, and calcium are associated with periodontitis in a nationally representative sample of Korean adults. This study comprised 6,150 adults 19 or more years old who took both periodontal examination and nutrition survey. The frequency of yogurt and milk intake was examined with a food frequency questionnaire. The amount of calcium intake was calculated with dietary intakes data gained from complete one-day 24-hour recall interviews. Periodontitis was assessed using the Community Periodontal Index (CPI). Multivariate logistic regression analyses were performed for the whole sample and subgroups with the strata of age, gender, or smoking, in a complex sampling design. Less intake of yogurt was significantly associated with periodontitis (odds ratio [OR] 0.82, 95% confidential interval [CI] 0.70–0.97), but neither less intake of milk (OR 1.04, 95% CI 0.89–1.20) nor lower intake of calcium (OR 1.04, 95% CI 0.89–1.21) was significantly associated with periodontitis. In the subgroup analysis, no difference in the association of yogurt intake with periodontitis was found according to the strata of age, gender, and smoking. In conclusion, periodonitis was significantly associated with the less intake of yogurt among the Korean adults, but the calcium contained in yogurt is not likely to cause it.

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Visual census and hydro-acoustic survey was carried out at Ulju small scale marine ranching area (MRA) to estimate demersal fish aggregations on September and November 2013. In this hydro-acoustic survey, the authors combined an image sonar with a scientific echo sounder to monitor an underwater situation and compare two acoustic data. Consequently, visual census survey was useful to estimate fish species composition for hydro-acoustic survey, because it is easy to identify aggregated fish species and overcome limits on a fishing depth and ability of an conventional fishing gear like a bottom gill-net or a fish trap at marine ranching area. Mean fish density was estimated as 0.757 g/m 2 on September and 0.219 g/m 2 on November and Fish abundance was finally calculated as 1.51ton (coefficient of variation, CV=13.1%) on September and 0.44ton (CV=47.7%) on November, respectively. Hydro-acoustic survey combined with the image sonar was useful to monitor fish aggregations and estimate fish stocks around artificial reefs at shallow coastal MRA. We were able to easily identify the underwater structures like an artificial reef and a fishing rope as well as fish aggregations from image sonar data. Therefore, the method was effective to separate unwanted echo signals in acoustic data of scientific echo sounder.