The lower Pliocene Bouse Formation in the lower Colorado River Valley (southwestern USA) consists of basal marl and dense tufa overlain by siltstone and fi ne sandstone. It is locally overlain by and interbedded with sands derived from the Colorado River. We briefl y review 87 Sr/ 86 Sr analyses of Bouse carbonates and shells and carbonate and gypsum of similar age east of Las Vegas that indicate that all of these strata are isotopically similar to modern Colorado River water. We also review and add new data that are consistent with a step in Bouse Formation maximum elevations from 330 m south of Topock Gorge to 555 m to the north. New geochemical data from glass shards in a volcanic ash bed within the Bouse Formation, and from an ash bed within similar deposits in Bristol Basin west of the Colorado River Valley, indicate correlation of the two ash beds and coeval submergence of both areas. The tuff bed is identifi ed as the 4.83 Ma Lawlor Tuff derived from the San Francisco Bay region. We conclude, as have some others, that the Bouse Formation was deposited in lakes produced by fi rst-arriving Colorado River water that entered closed basins inherited from Basin and Range extension, and estimate that fi rst arrival of river water occurred ca. Ma. If this interpretation is correct, addition of BristolBasin to the Blythe Basin inundation area means that river discharge was suffi cient to fi ll and spill a lake with an area of ~10,000 km 2 . For spillover to occur, evaporation rates must have been signifi cantly less in early Pliocene time than modern rates of ~2-4 m/yr, and/or Colorado River discharge was signifi cantly greater than the current ~15 km 3 /yr. In this lacustrine interpretation, evaporation rates were suffi cient to concentrate salts to levels that were hospitable to some marine organisms presumably introduced by birds.

We report strontium isotopic results for the late Miocene Hualapai Limestone of the Lake Mead area (Arizona-Nevada) and the latest Miocene to early Pliocene Bouse Formation and related units of the lower Colorado River trough (Arizona-CaliforniaNevada), together with parallel oxygen and carbon isotopic analyses of Bouse samples, to constrain the lake-overfl ow model for integration of the Colorado River. Sr iso topic analyses on the basal 1-5 cm of marl, in particular along a transect over a range of altitude in the lowest-altitude basin that contains freshwater, brackish, and marine fossils, document the 87 Sr/ 86 Sr of fi rst-arriving Bouse waters. Results reinforce the similarity between the 87 Sr/ 86 Sr of Bouse Formation carbonates and present-day Colorado River water, and the systematic distinction of these values from Neogene marine Sr. Basal Bouse samples show that 87 Sr/ 86 Sr decreased from 0.7111 to values in the range 0.7107-0.7109 during early basin fi lling. 87 Sr/ 86 Sr values from a recently identifi ed marl in the Las Vegas area are within the range of Bouse Sr ratios. 87 Sr/ 86 Sr values from the Hualapai Limestone decrease upsection from 0.7195 to 0.7137, in the approach to a time soon after 6 Ma when Hualapai deposition ceased and the Colorado River became established through the Lake Mead area. Bouse Formation δ 18 O values range from -12.9‰ to +1.0‰ Vienna Pee Dee belemnite (VPDB), and δ 13 C between -6.5‰ and +3.4‰ VPDB. Negative δ 18 O values appear to require a continental origin for waters, and the trend to higher δ 18 O suggests evaporation in lake waters.Sr and stable isotopic results for sectioned barnacle shells and from bedding planes of the marine fi sh fossil Colpichthys regis demonstrate that these animals lived in saline freshwater, and that there is no evidence for incursions of marine water, either long-lived or brief in duration. Lack of correlation of Sr and O isotopic variations in the same samples also argue strongly against systematic replacement of Sr in Bouse carbonates after deposition. Our results reinforce the conclusion that the Bouse Formation was deposited in a descending series of basins connected by overfl ow of Colorado River water. The Hualapai Limestone records a separate and earlier lake that may have been progressively infl uenced by Colorado River water as the time of river integration approached.