BackgroundAnemia among Women of Reproductive Age (WRA) continues to be among the major public health problems in many developing countries, including Rwanda, where it increased in prevalence between the 2015 and 2010 Rwanda Demographic and Health Survey (RDHS) reports. A thorough understanding of its risk factors is necessary to design better interventions. However, to the best of our knowledge, no study has been conducted in Rwanda on a nationally representative sample to assess factors associated with anemia among WRA. Accordingly, this study was conducted to address such gap.MethodsA quantitative, cross-sectional study was conducted using data from the RDHS 2014–2015. The study population consisted of 6680 WRA who were tested for anemia during the survey. Anemia was defined as having a hemoglobin level equal to or below 10.9 g/dl for a pregnant woman, and hemoglobin level equal to or below 11.9 g/dl for a non-pregnant woman. Pearson’s chi-squared test and multiple logistic regression were conducted for bivariate and multivariable analysis, respectively.ResultsThe prevalence of anemia among WRA was 19.2% (95% CI: 18.0–20.5). Four factors were found to be associated with lower odds of anemia, including being obese (OR: 0.61, 95% CI: 0.40–0.91), being in the rich category (OR: 0.74, 95% CI: 0.63–0.87), sleeping under a mosquito net (OR: 0.85, 95% CI: 0.74–0.98), and using hormonal contraceptives (OR: 0.61, 95% CI: 0.50–0.73). Five factors were associated with higher odds of anemia, including being underweight (OR: 1.39, 95% CI: 1.09–1.78), using an intrauterine device (OR: 1.98, 95% CI: 1.05–3.75), being separated or widowed (OR: 1.35, 95% CI: 1.09–1.67), and living in the Southern province (OR: 1.45, 95% CI: 1.11–1.89) or in the Eastern province (OR: 1.41, 95% CI: 1.06–1.88).ConclusionAnemia continues to pose public health challenges; novel public health interventions should consider geographic variations in anemia risk, seek to improve women’s economic statuses, and strengthen iron supplementation especially for Intrauterine device users. Additionally, given the association between anemia and malaria, interventions to prevent malaria should be enhanced.

BackgroundThe One Health (OH) concept, formerly referred to as ‘One Medicine’ in the later part of the 20th century, has gained exceptional popularity in the early 21st century, and numerous academic and non-academic institutions have developed One Health programs.ObjectivesTo summarize One Health training, research, and outreach activities originating in North America.MethodsWe used data from extensive electronic records maintained by the One Health Commission (OHC) (www.onehealthcommission.org/) and the One Health Initiative (www.onehealthinitiative.com/) and from web-based searches, combined with the corporate knowledge of the authors and their professional contacts. Finally, a call was released to members of the OHC's Global One Health Community listserv, asking that they populate a Google document with information on One Health training, research, and outreach activities in North American academic and non-academic institutions.ResultsA current snapshot of North American One Health training, research, and outreach activities as of August 2016 has evolved.ConclusionsIt is clear that the One Health concept has gained considerable recognition during the first decade of the 21st century, with numerous current training and research activities carried out among North American academic, non-academic, government, corporate, and non-profit entities.

While the distribution of RNA polymerase II (PolII) in a variety of complex genomes is correlated with gene expression, the presence of PolII at a gene does not necessarily indicate active expression. Various patterns of PolII binding have been described genome wide; however, whether or not PolII binds at transcriptionally inactive sites remains uncertain. The two X chromosomes in female cells in mammals present an opportunity to examine each of the two alleles of a given locus in both active and inactive states, depending on which X chromosome is silenced by X chromosome inactivation. Here, we investigated PolII occupancy and expression of the associated genes across the active (Xa) and inactive (Xi) X chromosomes in human female cells to elucidate the relationship of gene expression and PolII binding. We find that, while PolII in the pseudoautosomal region occupies both chromosomes at similar levels, it is significantly biased toward the Xa throughout the rest of the chromosome. The general paucity of PolII on the Xi notwithstanding, detectable (albeit significantly reduced) binding can be observed, especially on the evolutionarily younger short arm of the X. PolII levels at genes that escape inactivation correlate with the levels of their expression; however, additional PolII sites can be found at apparently silenced regions, suggesting the possibility of a subset of genes on the Xi that are poised for expression. Consistent with this hypothesis, we show that a high proportion of genes associated with PolII-accessible sites, while silenced in GM12878, are expressed in other female cell lines.

Background Anemia among Women of Reproductive Age (WRA) continues to be among the major public health problems in many developing countries, including Rwanda, where it increased in prevalence between the 2015 and 2010 Rwanda Demographic and Health Survey (RDHS) reports. A thorough understanding of its risk factors is necessary to design better interventions. However, to the best of our knowledge, no study has been conducted in Rwanda on a nationally representative sample to assess factors associated with anemia among WRA. Accordingly, this study was conducted to address such gap. Methods A quantitative, cross-sectional study was conducted using data from the RDHS 2014-2015 report. The study population consisted of 6680 WRA who were tested for anemia during the survey. Anemia was defined as having a hemoglobin level equal to or below 10.9 g/dl for a pregnant woman, and hemoglobin level equal to or below 11.9 g/dl for a non-pregnant woman. Pearson’s chi-squared test and multiple logistic regression were conducted for bivariate and multivariable analysis, respectively. Results The prevalence of anemia among WRA was 19.2% (95% CI: 18.0 - 20.5). Four factors were found to be associated with lower odds of anemia, including being obese (OR: 0.61, 95% CI: 0.40 - 0.91), being in the rich category (OR: 0.74, 95% CI: 0.63 - 0.87), sleeping under a mosquito net (OR: 0.85, 95% CI: 0.74 - 0.98), and using hormonal contraceptives (OR: 0.61, 95% CI: 0.50 - 0.73). Five factors were associated with higher odds of anemia, including being underweight (OR: 1.39, 95% CI: 1.09 - 1.78), using an intrauterine device (OR: 1.98, 95% CI: 1.05 - 3.75), being separated or widowed (OR: 1.35, 95% CI: 1.09 - 1.67), and living in the Southern province (OR: 1.45, 95% CI: 1.11 - 1.89) or in the Eastern province (OR: 1.41, 95% CI: 1.06 - 1.88). Conclusion Anemia continues to pose public health challenges; novel public health interventions should consider geographic variations in anemia risk, seek to improve women’s economic statuses, and strengthen iron supplementation especially for Intrauterine device users. Additionally, given the association between anemia and malaria, interventions to prevent malaria should be enhanced.

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Individuals living with hypertension are predisposed to higher risk of stroke, kidney diseases and heart failure. Approximately 9.4 million people worldwide die from complications related to hypertension every year. Hypertension is often known as the silent killer because many people do not develop any symptoms until they get very sick. Early screening is particularly important for better treatment outcomes yet it remains a challenge in many countries. Worldwide, approximately 50% of people are living with undiagnosed hypertension. In Rwanda, the rate of undiagnosed hypertension is unknown, and so are the associated risk factors in rural communities. A cross-sectional descriptive study was conducted to determine the rate and risk factors of undiagnosed hypertension among adults in a rural community in Rwanda.  The proportion of people having undiagnosed hypertension was found to be high. Out of 155 study participants, 41.9% had undiagnosed hypertension, with slightly more men having hypertension (52.3%) than women (47.7%). More than 98% of respondents either did not know or knew wrong information about hypertension, and only 3% knew they should have regular checkups with physicians. Age (p=0.027) and alcohol consumption (p=0.014) were found to be statistically significantly associated with hypertension. Smoking and exercise were not found to be risk factors as most Rwandans living in the rural areas are physically active.  Programs to promote hypertension awareness, encourage regular physical checkups, and reduce alcohol consumption are needed to improve diagnosis and control of hypertension in Rwanda. Community programs offering free regular blood pressure checks may also be helpful in identifying early hypertension. Larger scale studies of this kind should be conducted to understand whether results can be generalized to other areas of Rwanda.

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Plagiarism is a serious type of scholastic misconduct. In Rwanda, no research has been conducted to assess university students’ attitudes and knowledge of plagiarism and if they have the skills to avoid plagiarizing. This study was conducted to assess knowledge of and attitudes towards plagiarism, as well as ability to recognize plagiaristic writing, among university students in Rwanda. An online questionnaire containing 10 knowledge questions, 10 attitude statements, and 5 writing cases with excerpts to test identification of plagiarism was administered between February and April 2021. Out of the 330 university students from 40 universities who completed the survey, 75.8% had a high knowledge level (score ≥ 80%), but only 11.6% had a high score in recognizing plagiaristic writing (score ≥ 80%). There was no statistically significant association between knowledge level and ability to recognize plagiaristic writing ( P = 0.109). Lower odds were found in both diploma/certificate and bachelor students of having high knowledge as well as of having high ability to recognize plagiaristic writing than in master’s students. Although respondents generally disapproved of plagiarism, approximately half of the respondents indicated that sometimes plagiarism is unavoidable, and self-plagiarism should not be punished in the same way as plagiarism of others’ work. Inter-collegial collaboration on effective plagiarism policies and training programs is needed.