Photosystem II (PSII), the light-driven water/plastoquinone photooxidoreductase, is of central importance in the planetary energy cycle. The product of the reaction, plastohydroquinone (PQH2), is released into the membrane from the QB site, where it is formed. A plastoquinone (PQ) from the membrane pool then binds into the QB site. Despite their functional importance, the thermodynamic properties of the PQ in the QB site, QB, in its different redox forms have received relatively little attention. Here we report the midpoint potentials (Em) of QB in PSII from Thermosynechococcus elongatus using electron paramagnetic resonance (EPR) spectroscopy: Em QB/QB•− ≈ 90 mV, and Em QB•−/QBH2 ≈ 40 mV. These data allow the following conclusions: 1) The semiquinone, QB•−, is stabilized thermodynamically; 2) the resulting Em QB/QBH2 (∼65 mV) is lower than the Em PQ/PQH2 (∼117 mV), and the difference (ΔE ≈ 50 meV) represents the driving force for QBH2 release into the pool; 3) PQ is ∼50× more tightly bound than PQH2; and 4) the difference between the Em QB/QB•− measured here and the Em QA/QA•− from the literature is ∼234 meV, in principle corresponding to the driving force for electron transfer from QA•− to QB. The pH dependence of the thermoluminescence associated with QB•− provided a functional estimate for this energy gap and gave a similar value (≥180 meV). These estimates are larger than the generally accepted value (∼70 meV), and this is discussed. The energetics of QB in PSII are comparable to those in the homologous purple bacterial reaction center.

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A free-space optical (FSO) communication demonstration was conducted with JHU/APL and AOptix at the TCOM Test Facility in Elizabeth City, NC in May 2006. The primary test objective was to evaluate the performance of an FSO link from a fiber-tethered aerostat to a ground platform at effective data rates approaching 100 Gigabits/sec using wavelength division multiplexing (WDM) techniques. (Multiple optical channels operating near 1550 nm were modulated at data rates of 1, 10 and 40 Gbps). The test was conducted with a 38 meter aerostat raised to an altitude of 1 km and a ground platform located 1.2 km from the aerostat (limited by property boundary). Error free data transfers of 1.2 Terabits in 30 seconds at 40 Gbps were demonstrated. The total data transferred during the test was greater than 30 Terabits with an average BER of 10 -6 without any forward error correction (FEC) coding.

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