Chronic or recurrent inflammation plays a role in the development of many types of cancer including prostate cancer. CXCL10 (interferon-gamma inducible protein-10, IP-10) is a small secretory protein of 8.7 kDa. Recently, it has been shown that normal prostate epithelial (PZ-HPV-7) cells produce lower amounts of angiogenic CXC chemokines (GRO-alpha, IL-8) and higher amounts of angiostatic chemokines (CXCL10, CXCL11) as compared to prostate cancer cells (CA-HPV-10 and PC-3). Accordingly, we studied the effects of overexpression of CXCL10 in human prostate cancer LNCaP cells. LNCaP cells were transiently transfected with CXCL10 cDNA in pIRES2-EGFP vector. CXCL10, CXCR3, PSA and G3PDH mRNA levels were determined by semi-quantitative conventional and quantitative real-time RT-PCR and fluorescence-activated cell sorting (FACS). The expression of CXCL10 was markedly enhanced in the transfected cells at mRNA and protein levels in the cells. Overexpression of CXCL10 inhibited cell proliferation of the transfected cells by 30%-40% in serum-limited medium (1% FCS in RPMI1640 medium) and decreased PSA production. CXCR3 expression was significantly induced by the overexpression of CXCL10 as determined by RT-PCR and FACS. These results indicated that CXCL10 inhibited LNCaP cell proliferation and decreased PSA production by up-regulation of CXCR3 receptor. CXCL10 may be potentially useful in the treatment of prostate cancer.
A need exists to prolong the release of rapidly metabolized peptides of a low molecular weight, while delivering this peptide without environmental interference. Previous studies have used bovine serum albumin (BSA) as a model peptide to study release characteristics from alginate microcapsules. BSA is 66 kDa in size, while the peptide of interest here, connexin-43 carboxyl-terminus mimetic peptide (αCT1), is only 3.4 kDa. Such a change in size results in a much different set of release parameters. Our overall goal is a sustained release over a 24+ h period. Prolonged application of the peptide to a wound site to investigate therapeutic effects is ideal. As a result, a diffusion method using alginate microcapsules, along with the addition of poly-l-lysine and poly-l-ornithine, has been explored. We first aimed to establish and characterize our parameters through a set of parametric tests. Variations in polymer coating, change in pH, and changes in loading ratio have previously been shown to effect release using model compounds. Here we test specific changes in these parameters to show effects on the release of αCT1. Additionally, the microcapsules were attached to several biomaterials and surgical implants by ultraviolet cross-linking to study the effectiveness of attachment and delivery. Analysis and measurements using phase contrast microscopy, scanning electron microscopy, and atomic force microscopy were used to characterize changes in microcapsule morphology.
We apply matrix theory over F 2 to understand the nature of socalled "successful pressing sequences" of black-and-white vertex-colored graphs. These sequences arise in computational phylogenetics, where, by a celebrated result of Hannenhalli and Pevzner, the space of sortingsby-reversal of a signed permutation can be described by pressing sequences. In particular, we offer several alternative linear-algebraic and graph-theoretic characterizations of successful pressing sequences, describe the relation between such sequences, and provide bounds on the number of them. We also offer several open problems that arose as a result of the present work.MSC classes: 05C50, 15B33, 92D15.
Juggling patterns can be described by a closed walk in a (directed) state graph, where each vertex (or state) is a landing pattern for the balls and directed edges connect states that can occur consecutively. The number of such patterns of length n is well known, but a long-standing problem is to count the number of prime juggling patterns (those juggling patterns corresponding to cycles in the state graph). For the case of b = 2 balls we give an expression for the number of prime juggling patterns of length n by establishing a connection with partitions of n into distinct parts. From this we show the number of two-ball prime juggling patterns of length n is γ −o(1) 2 n where γ = 1.32963879259 . . .. For larger b we show there are at least b n−1 prime cycles of length n.
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