Platelet-rich plasma (PRP) has received increasing attention and is widely used in clinical practice in order to stimulate human tissue healing. Contusions are very common injuries observed in sports and affect the function of the musculoskeletal system. This study investigated the effects of PRP on the oxidative damage determined by a contusion induced in gastrocnemius muscle of rats. PRP was injected intramuscularly immediately after injury and every 48 h, and the biochemical analysis was performed 1, 3, 5, or 7 days after the contusion onset in order to evaluate the changes characteristics of the healing process. The contusion increased the levels of oxidative stress markers such as thiobarbituric acid reactive substances and oxidized dichlorofluorescein both in skeletal muscle tissue and erythrocytes preparations, and PRP treatment significantly reduced these oxidative damage markers. Furthermore, the contusion decreased the cellular viability in the site of the lesion and PRP was effective in diminishing this effect. Moreover, PRP increased the levels of enzymatic antioxidants superoxide dismutase and catalase activities in the injured muscle, and also the non-protein thiols (-SH) group levels in erythrocytes. In conclusion PRP, in the form that was used in this study, was able to modulate the oxidative damage determined by a classical skeletal muscle injury possibly by reducing the impairment of myocytes mitochondrial function and improving their endogenous antioxidant defense systems.
Introdução-As fraturas cominutivas da diáfise umeral são muito freqüentes em acidentes. Como principais complicações, citamse as lesões do nervo radial. Objetivo-descrever os resultados da intervenção fisioterapêutica com a Estimulação Elétrica Funcional (FES), estímulos proprioceptivos e cinesioterapia progressiva em uma paciente com lesão do nervo radial, após fratura cominutiva de úmero. Metodologia-estudo de caso com análise descritiva. A avaliação foi composta por uma ficha de anamnese e exame físico. O tratamento, composto por 2 sessões semanais, com duração de 1 hora cada, totalizando 16 sessões, incluía estimulação da inervação, exercícios progressivos de fortalecimento muscular e funcionais. Resultados-paciente apresentou aumento de força muscular, da Amplitude de Movimento (ADM), principalmente de punho e articulação Metacarpofalangeana (MCF). O uso da FES mostrouse eficaz na estimulação da musculatura extensora do punho. Conclusão-a utilização da eletroestimulação e cinesioterapia progressiva apresentaram resultados positivos no tratamento dessa paciente, restaurando a sua ADM e força muscular.
Quercetin is a flavonoid with antioxidant and anti-inflammatory potential. However, it has low skin permeation and stability. One way to improve this is to incorporate it into nanocarriers. This work aimed to develop and evaluate the stability of a nanostructured lipidic carrier with quercetin (NLC-Q) under different storage conditions for 30 th days and its incorporation in the gel for 60 th days. The NLC-Q were developed by the high shear rate method, and the physicochemical stability was performed at temperatures of 5, 25 and 40 o C on days 0, 7, 15, 22 and 30. The gel containing NLC-Q had its stability evaluated on days 1, 7, 15, 30, 45 and 60. The three environments' size, polydispersity index (PdI), zeta potential (ZP), content (%), and pH are within the recommended parameters. Unlike the climatic chamber, where NLC-Q increased size, PdI and quercetin content (%) were reduced throughout the experiment. In the gel with NLC-Q, pH, viscosity, spreadability, size and PdI are within recommendations found in the literature. The NLC-Q kept at room temperature showed good stability throughout the study.
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