The leaf disc transformation/regeneration system was modified for tomato (L. esculentum). Both leaf explants and cotyledon/hypocotyl sections can be used to regenerate transformed plants. We have obtained over 300 transgenic plants from eight tomato cultivars. We have evidence for both single and multi-copy insertions of the T-DNA, and have demonstrated inheritance of the T-DNA insert in the expected Mendelian ratios. Several heterologous promoters function in tomato. A reduced efficiency of transformation was observed with binary T-DNA vectors as compared to co-integrate T-DNA vectors. The ease of the leaf disc method makes tomato a premier experimental organism for plant biotechnology.
A pea nuclear gene encoding the small subunit of ribulose-1,5-bisphosphate carboxylase was inserted into the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens and transferred into petunia cells by in vitro transformation. The transferred pea rbcS gene is expressed in petunia cells under the transcriptional control of its own promoter in a light-dependent fashion similar to that observed in pea leaves. In contrast, a nonphotosynthetic chimeric gene containing a nopaline synthase promoter is expressed constitutively in both light- and dark-grown tissues. In the transformed cells, transcripts from the pea rbcS gene are processed correctly and translated to yield an authentic pea small subunit polypeptide which is localized in chloroplasts.
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