No abstract

Semiconductor photocatalysis has been applied to the remediation of an extensive range of chemical pollutants in water over the past 30 years. The application of this versatile technology for removal of micro-organisms and cyanotoxins has recently become an area that has also been the subject of extensive research particularly over the past decade. This paper considers recent research in the application of semiconductor photocatalysis for the treatment of water contaminated with pathogenic micro-organisms and cyanotoxins. The basic processes involved in photocatalysis are described and examples of recent research into the use of photocatalysis for the removal of a range of microorganisms are detailed. The paper concludes with a review of the key research on the application of this process for the removal of chemical metabolites generated from cyanobacteria.

CopyrightItems in 'OpenAIR@RGU', Robert Gordon University Open Access Institutional Repository, are protected by copyright and intellectual property law. If you believe that any material held in 'OpenAIR@RGU' infringes copyright, please contact openair-help@rgu.ac.uk with details. The item will be removed from the repository while the claim is investigated. The mechanism of this process has been reported to involve attack by valence band generated hydroxyl radicals. In this study when three common bacterial pathogens, Escherichia coli, Salmonella enterica serovar Enteritidis and Pseudomonas aeruginosa, were exposed to TiO 2 and UVA light a substantial decrease in bacterial numbers was observed. Control experiments in which all three pathogens were exposed to UVA light only resulted in a similar reduction in bacterial numbers.Moreover exposure to UVA light alone resulted in the production of a smaller than average colony phenotype among the surviving bacteria, for all three pathogens examined, a finding which was not observed following treatment with UVA and Variants of pathogenic bacteria following UVA treatment of drinking water may represent a health hazard. As these Small Colony Variants were not observed with the UVA/TiO 2 system this potential hazard is not a risk when using this technology. It would also appear the bactericidal mechanism is different with the UVA/TiO 2 process compared to when UVA light is used alone.2

The current study sought to assess the importance of three common variables on the outcome of TiO(2) photocatalysis experiments with bacteria. Factors considered were (a) ability of test species to withstand osmotic pressure, (b) incubation period of agar plates used for colony counts following photocatalysis and (c) chemical nature of suspension medium used for bacteria and TiO(2). Staphylococcus aureus, Escherichia coli, Salmonella ser. Typhimurium and Pseudomonas aeruginosa were found to vary greatly in their ability to withstand osmotic pressure, raising the possibility that osmotic lysis may be contributing to loss of viability in some photocatalytic disinfection studies. Agar plate incubation time was also found to influence results, as bacteria treated with UV light only grew more slowly than those treated with a combination of UV and TiO(2.) The chemical nature of the suspension medium used was found to have a particularly pronounced effect upon results. Greatest antibacterial activity was detected when aqueous sodium chloride solution was utilised, with approximately 1 x 10(6) CFU mL(-1)S. aureus being completely killed after 60 min. Moderate activity was observed when distilled water was employed with bacteria being killed after 2h and 30 min, and no antibacterial activity at all was detected when aqueous tryptone solution was used. Interestingly, the antibacterial activity of UV light on its own appeared to be very much reduced in experiments where aqueous sodium chloride was employed instead of distilled water.

The roles of flagella and five fimbriae (SEF14, SEF17, SEF21, pef, lpf) in the early stages (up to 3 days) of Salmonella enterica serovar Enteritidis (S. Enteritidis) infection have been investigated in the rat. Wild-type strains LA5 and S1400 (fim+/fla+) and insertionally inactivated mutants unable to express the five fimbriae (fim2/fla+), flagella (fim+/fla2) or fimbriae and flagella (fim2/fla2) were used. All wild-type and mutant strains were able to colonize the gut and spread to the mesenteric lymph nodes, liver and spleen. There appeared to be little or no difference between the fim2/fla+ and wild-type (fim+/fla+) strains. In contrast, the numbers of aflagellate (fim+/fla2 or fim2/fla2) salmonella in the liver and spleen were transiently reduced. In addition, fim+/fla2 or fim2/fla2 strains were less able to persist in the upper gastrointestinal tract and the inflammatory responses they elicited in the gut were less severe. Thus, expression of SEF14, SEF17, SEF21, pef and lpf did not appear to be a prerequisite for induction of S. Enteritidis infection in the rat. Deletion of flagella did, however, disadvantage the bacterium. This may be due to the inability to produce or release the potent immunomodulating protein flagellin.

No abstract

Rat ileal air interface and submerged explant models were developed and used to compare the adhesion of Salmonella enterica var Enteritidis wild-type strains with that of their isogenic single and multiple deletion mutants. The modi®ed strains studied were defective for ®mbriae,¯agella, motility or chemotaxis and binding was assessed on tissues with and without an intact mucus layer. A multiple a®mbriate=a¯agellate (®m 2 =¯a 2 ) strain, a ®mbriate but a¯agellate (¯a 2 ) strain and a ®mbriate=¯agellate but non-motile (mot 2 ) strain bound signi®cantly less extensively to the explants than the corresponding wild-type strains. With the submerged explant model this difference was evident in tissues with or without a mucus layer, whereas in the air interface model it was observed only in tissues with an intact mucus layer. A smooth swimming chemotaxis-defective (che 2 ) strain and single or multiple a®mbriate strains bound to explants as well as their corresponding wild-type strain. This suggests that under the present experimental conditions ®mbriae were not essential for attachment of S. enterica var Enteritidis to rat ileal explants. However, the possession of active¯agella did appear to be an important factor in enabling salmonellae to penetrate the gastrointestinal mucus layer and attach speci®cally to epithelial cells.

Photocatalytic oxidation of coumarin to 7-hydroxycoumarin was used in order to identify the optimum conditions and the potential limitations of a photocatalytic screening method.