Simple methods for the titration of rabbit C1 and C5-9 have been developed which do not require purified complement components. The stable cellular intermediate, sheep erythrocyte-rabbit antibody-bovine complement C4 and C2 (SHEACBo42) is used for these assay methods with kinetic studies on the interaction between rabbit C1 and C5-9 with SHEACBo42 forming the basis for the methods. Furthermore, these assay methods have many applications for the evaluation of in vivo complement activation of both the classical and alternate pathways.
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