Palindromic peptide LfcinB (21–25)Pal: RWQWRWQWR was synthesized by Solid Phase Peptide Synthesis (SPPS‐Fmoc/tBu), purified by Reverse Phase Solid Phase Extraction (RP‐SPE) and characterized by Reverse Phase High Performance Liquid Chromatography (RP‐HPLC) and Matrix‐Assisted Laser Desorption/Ionization‐Time Of Flight Mass Spectrometry (MALDI‐TOF MS). The antifungal activity of LfcinB (21–25)Pal against both ATCC strains and clinical isolates of C. albicans, C. glabrata, C. krusei, C. auris and C. tropicalis was evaluated. The palindromic peptide exhibited fungistatic and fungicidal activity against all yeast evaluated. The antifungal activity was dependent on peptide concentration in all cases. Additionally, LfcinB (21–25)Pal (25–50 μg/mL) combined with fluconazole exhibited a synergistic antifungal effect against C. tropicalis 883 and C. krusei 6258 (resistant to fluconazole). This study showed that the palindromic peptide derived from Lactoferricin B (LfcinB) exhibited significant antifungal activity against Candida spp, suggesting that this peptide could have a therapeutic application solely or in combination with fluconazole.
ResumenEl objetivo de esta investigación fue implementar un método microbiológico que permita reducir las concentraciones de cromo (VI) de lodos residuales provenientes de curtiembres mediante la combinación de procesos de ingeniería ambiental y microbiología. Se empleó un sistema de medio fijo que contenía un soporte de plástico al cual se adhiere la lama de microorganismos que realiza el proceso de purificación bajo condiciones físicas controladas, esto es demostrado in vivo con mediciones de cromo VI por absorción atómica del efluente tratado e in vitro mediante aislamiento e identificación de los microorganismos en medios de cultivo selectivos con K 2 CrO 4 . Los resultados demuestran una reducción de Cr (VI) del 58% por parte de los microorganismos: Bacillus cereus, Acinetobacter iwoffi, Lactobacillus agilis, Penicillium sp. y Cladosporium sp. capaces de tolerar concentraciones de 300 ppm de Cr (VI).Palabras clave: cromo, curtiembres, microorganismos biorremediadores, sistema de medio fijo.Evaluation of a system of fixed media support for a film capable of reducing microbial Cr (VI) of tannery sewage sludge Abstract The goal of this research was to implement a microbiological method to reduce the concentrations of chromium (VI) of sewage sludge from tanneries through the combination of processes of environmental engineering and microbiology. A system of fixed medium was used, it contained a plastic support to which the lama of microorganisms adheres, performing the purification process under controlled physical conditions, this is demonstrated in vivo with measurements of chromium VI by atomic absorption of treated effluent and in vitro by isolation and identification of microorganisms in selective culture media with K2CrO4. The results show a 58% Cr (VI) reduction of the microorganisms: Bacillus cereus, Acinetobacter iwoffi, Lactobacillus agilis, Penicillium sp. and Cladosporium sp. Which are able to tolerate Cr (VI) concentrations of 300 ppm.
Dossier CieNCiAs BÁsiCAs, MeDiCiNA orAL, BioTeCNoLoGÍA Y BioiNForMÁTiCA eN oDoNToLoGÍA RESUMEN Antecedentes: la melatonina es una hormona producida en organismos vertebrados, invertebrados y plantas. Tiene importancia odontológica por su participación en procesos de oseointegración e inmunomodulación. Además, se ha reportado una posible actividad antibacteriana. Objetivo: evaluar el efecto antibacteriano de la melatonina sobre Streptococcus mutans CIO315, Staphylococcus aureus CIO613, Staphylococcus epidermidis CIO615 y Escherichia coli CIO465. Métodos: este estudio de diseño experimental se llevó a cabo con el método de dilución en agar Mueller-Hinton. Se evaluaron concentraciones de melatonina de 43 a 3 µmol. De cada una de las cuatro bacterias se preparó una suspensión que se ajustó a la escala 0,5 de McFarland. Finalmente, sobre la superficie del agar se colocó 1 µl de cada bacteria y se incubó por 24 y 48 h a 37 °C. Resultados: la melatonina mostró un efecto antibacteriano frente a los cuatro microrganismos estudiados en una concentración de 43 µmol a las 24 y 48 h. Conclusiones: la melatonina presenta una excelente actividad antimicrobiana sobre Streptococcus mutans CIO315, Staphylococcus aureus CIO613, Staphylococcus epidermidis CIO615 y Escherichia coli CIO465 en una concentración de 43 µmol, lo que indica que se podría utilizar como una opción en la prevención y tratamiento de patologías orales como la caries dental y la periodontitis. PALABRAS CLAVE Antibacteriano; Escherichia coli; melatonina; metabolismo óseo; Staphylococcus aureus; Staphylococcus epidermidis; Streptococcus mutans ÁREA TEMÁTICA Microbiología oral ABSTRACT Background:Melatonin is a hormone produced by vertebrate organisms, invertebrates, and plants. It is important in dentistry because it participates in osseointegration and immunomodulation processes. In addition, there are reports suggesting a possible antibacterial activity of melatonin. Purpose: To evaluate the antibacterial effect of melatonin on Streptococcus mutans CIO315, Staphylococcus aureus CIO613, Staphylococcus epidermidis CIO615, and Escherichia coli CIO465. Methods: This experimental study was carried out through the Mueller-Hinton agar dilution method. 43 to 3 µmol melatonin concentrations were analyzed on a 0.5 McFarland adjusted suspension of each strain. Finally a 1 µl of each microorganism was spread on the agar and incubated at 37 °C for 24 and 48 h. Results: Melatonin showed an antibacterial effect against the four bacteria studied with a 43 µmol mmolconcentration at 24 and 48 h. Conclusions: Melatonin has excellent antimicrobial activity against Streptococcus mutans CIO315, Staphylococcus aureus CIO613, Staphylococcus epidermidis CIO615, and Escherichia coli CIO465 at a concentration of 43 µmol, indicating that it could be an option in the prevention and treatment of oral diseases such as dental caries and periodontitis.
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