The purpose of this research was to improve probiotic microencapsulation using prebiotics and : The purpose of this research was to improve probiotic microencapsulation using prebiotics and : The purpose of this research was to improve probiotic microencapsulation using prebiotics and : The purpose of this research was to improve probiotic microencapsulation using prebiotics and : The purpose of this research was to improve probiotic microencapsulation using prebiotics and modern optimization techniques to determine optimal processing conditions, performance, and survival rates. modern optimization techniques to determine optimal processing conditions, performance, and survival rates. modern optimization techniques to determine optimal processing conditions, performance, and survival rates. modern optimization techniques to determine optimal processing conditions, performance, and survival rates. modern optimization techniques to determine optimal processing conditions, performance, and survival rates. Prebiotics (fructooligosaccharides or isomaltooligosaccharides), growth promoter (peptide), and sodium algi-Prebiotics (fructooligosaccharides or isomaltooligosaccharides), growth promoter (peptide), and sodium algi-Prebiotics (fructooligosaccharides or isomaltooligosaccharides), growth promoter (peptide), and sodium algi-Prebiotics (fructooligosaccharides or isomaltooligosaccharides), growth promoter (peptide), and sodium algi-Prebiotics (fructooligosaccharides or isomaltooligosaccharides), growth promoter (peptide), and sodium alginate were incorporated as coating materials to microencapsulate 4 probiotics ( nate were incorporated as coating materials to microencapsulate 4 probiotics ( nate were incorporated as coating materials to microencapsulate 4 probiotics ( nate were incorporated as coating materials to microencapsulate 4 probiotics ( nate were incorporated as coating materials to microencapsulate 4 probiotics (Lactobacillus acidophilus Lactobacillus acidophilus Lactobacillus acidophilus Lactobacillus acidophilus Lactobacillus acidophilus, , , , , Lacto-Lacto-Lacto-Lacto-Lactobacillus casei bacillus casei bacillus casei bacillus casei Bifidobacterium longum). The proportion of the prebiotics, pep-). The proportion of the prebiotics, pep-). The proportion of the prebiotics, pep-). The proportion of the prebiotics, pep-). The proportion of the prebiotics, peptide, and sodium alginate was optimized using response surface methodology (RSM) to 1st construct a surface tide, and sodium alginate was optimized using response surface methodology (RSM) to 1st construct a surface tide, and sodium alginate was optimized using response surface methodology (RSM) to 1st construct a surface tide, and sodium alginate was optimized using response surface methodology (RSM) to 1st construct a surface tide, and sodium alginate was optimized using response surface methodology (RSM) to 1st construct a surface model, with sequential quadratic programming (SQP) subsequently adopted to optimize the model and evaluate model, with sequ...