A series of experiments is presented, describing the development of the rumen function in grazing lambs and the effects of' diet on the rumen development of artificially reared lambs. In grazing lambs the volume of the abomasum contents showed little change with age. The volume of the rumen contents increased with age, and the rate of' this increase was most marked from 3 weeks of age onwards. The volume of the rumen contents, relative to both the liveweight and the volume of the abomasum contents, was constant from 8 weeks of age onwards. The rumen volatile fatty acids (V.F.A.) concentrations in grazing lambs increased from birth and reached adult levels at 8 weeks of age. The total quantity of V.F.A. present in the rumen at a given time, relative to liveweight, increased up to 8 weeks of age but from this age onwards was approximately constant. The rumen ammonia nitrogen (N) concentrations in grazing lambs were high for the first few weeks after birth, but showed a continuous fall, reaching adult levels at 5 weeks of age. The quantity of ammonia N present in the rumen at a given time, relative to liveweight, increased up to 8 weeks of age and then remained approximately constant. The rumen volumes, V.F.A., and ammonia N concentrations were normal only in lambs that had been fed roughage. When lucerne chaff was fed to young lambs, the digestibilities of the dry matter, crude protein, and crude fibre were high, irrespective of whether the previous diet had been milk or chaff. The ad libitum dry matter intake from roughage, relative to the liveweight, increased up to 8 weeks of age and then remained approximately constant. When roughage was fed to lambs which had been reared on 'milk' (a preparation from dried cows' milk) only from birth to 9 weeks of age, the rumen V.F.A. concentrations rose immediately and levelled off at adult levels within 1 week.
1. When fractionated by sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE), strained rumen fluid from sheep fed on pelleted lucerne (Medicago sativa) hay showed no major protein components that stain with Coomassie Blue. This feature made it possible to monitor the fate of individual polypeptides within a protein mixture incubated in rumen fluid in vitro. 2. Extracts from a number of seed meals (sunflower (Helianthus annuus), lupin (Lupinus angustifolius), rape (Brassica napus) and pea (Pisum sativum L.)), as well as casein and bovine serum albumin, were examined in this system. The protein components of each seed type showed a wide range of resistances to degradation. One protein in pea seeds (pea albumin 1), which is particularly rich in cysteine, was almost as resistant to rumen degradation as bovine serum albumin. 3. Analysis of synthetic-fibre-bag experiments by SDS-PAGE showed that the rate of loss of total protein from solid meal residues does not provide an index of the resistance of individual protein components of the meal to rumen degradation. While there was no qualitative change in the protein profile of residual pea-seed meal inside a synthetic-fibre bag, there was considerable variation in the rate at which individual, solubilized protein components were degraded in the surrounding rumen fluid.
1. Thirty-two single lambs were grazed together on pasture. At weekly intervals, from birth to 112 days of age, two lambs were slaughtered and various organs weighed immediately after death. Also the alimentary tracts of seven adult sheep were obtained immediately after death.2. The rumen had the fastest growth rate of the four stomachs, followed by the reticulum, omasum, and abomasum, in that order.3. Immediately after birth the four stomachs altered rapidly in their relative proportions, but by about 66 days of age they had reached approximately adult proportions.4. The weights of the liver, heart and kidneys, relative to live weight, decreased during the first few weeks of life, and thereafter remained constant.5. The weight of the spleen, relative to live weight, increased during the first week of life and thereafter remained constant.
Three experiments with sheep were carried out to investigate the effect of urea, added to a diet of straw plus molasses, on roughage intake and digestion, and on the nitrogen status of the animal. Urea, added to straw and molasses at the level of 3% of the amount of straw, increased the ad libitum food intake, rate of cellulose (cotton thread) digestion in the rumen, and rate of passage of food through the gut. When different amounts of urea were fed, the highest levels of intake, rate of cellulose digestion, and rate of passage occurred with 8–16 g urea per sheep per day. Increasing the amount of urea fed to 32 g per day caused significant decreases in rate of passage and intake, within diets containing urea. These were not accompanied by significant changes in rate of cellulose digestion in the rumen. With diets of straw and molasses, with and without urea, crude fibre digestibility was positively correlated with the rate of cellulose digestion in the rumen. Voluntary intake of these diets was positively correlated with rate of passage. When the effect of rate of passage was eliminated, voluntary intake was not significantly correlated with the rate of cellulose digestion in the rumen. Under the feeding conditions used in these experiments, once sufficient urea had been added to the diet to bring the animal into a small, positive nitrogen balance, additional dietary nitrogen supplied as urea was practically all excreted in the urine. It is concluded that, under these feeding conditions, the primary function of a urea supplement is to enable an animal to maintain nitrogen equilibrium rather than store significant amounts of nitrogen in the body.
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