Conventional affinity biosensor typically relies on passive diffusion of analytes for binding reaction, which in many cases leads to long response time and lack of sensitivity. Recent research showed that directed particle motion towards sensor electrodes could be induced in sample matrix by applying an inhomogeneous AC electric field, often with AC dielectrophoresis as the responsible mechanism. As a result, shorter assay time and higher sensitivity can be achieved. Previously, we demonstrated a rapid and sensitive AC capacitive affinity sensor, which integrates low voltage AC dielectrophoresis into label-free capacitive measurement to achieve a single-step operation without any wash steps for clinical samples. However, dielectrophoretic force is rather short-ranged, and is also proportional to the size of target biomolecules/particles. Therefore, to detect target molecule at diluted concentrations or small molecule, improvement in sensitivity by dielectrophoresis could be quite limited. Alternatively, AC electric field can also produce microfluidic movement to carry biomolecules to sensors, which is of long range and size independent. This work demonstrates the use of low voltage AC electrothermal effect to enhance and accelerate the detection of low abundance and small target molecules by AC capacitive sensing with simultaneous AC electrokinetic enrichment. Electrode designs were studied for their effectiveness in AC electrothermal capacitive sensing. Electrodes with larger characteristic length were found to be more amenable to inducing AC electrothermal convection and were successfully used to detect low abundance protein and femto-molar level small molecules.

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We report a flexible sensor array electronic tongue system that is fabricated on a polymer substrate by the laser direct writing process for multiflavor detection. Electronic tongue is a sensing system that is applied to detect different elements with the same sensor array. By analyzing responses from different measurement units, it enables a cross-sensitivity, namely, the ability of the system to responding to a range of different analytes in solution without specific functionalization of sensors. In this article, a six-unit sensing array system was fabricated by a laser direct writing process. Sensing units were introduced on a flexible polyamide surface. A high surface-volume ratio porous carbon structure was created by a laser-induced carbonization process, which provides stable conductive carbon electrodes with high sensitivity. Different surface treatments, such as gold plating, reduced-graphene oxide coating, and polyaniline coating, were accomplished for different measurement units. By applying principal component analysis, this sensing system shows a promising result for the detection of multiple flavors. The detection limit for each element is about 0.1 mM for NaCl and sugar solutions. Also, it is able to detect 10 times diluted commercial table vinegar solution, which originally contains 5% acetic acid. The detection limit is theoretically lower than the human threshold of 10 mM for NaCl and sugar. Besides, the sensing system shows a high sensitivity and selectivity for mixed elements. By mapping the data points, the sensor system could detect flavor combinations and provide a reliable prediction of analyte concentration ratios.

Bisphenol A (BPA) is an endocrine disrupting compound that may have adverse developmental, reproductive, neurological, and immune system effects. Low-level exposure to BPA is ubiquitous in human populations due to its widespread use in consumer products. Therefore, highly sensitive methods are needed to quantify BPA in various matrices including water, serum, and food products. In this study, we developed a simple, rapid, highly sensitive and specific sensor based on an aptamer probe and AC electrokinetics capacitive sensing method that successfully detected BPA at femto molar (fM) levels, which is an improvement over prior work by a factor of 10. We were able to detect BPA spiked in human serum as well as in maternal and cord blood within 30s. The sensor is responsive to BPA down to femto molar levels, but not to structurally similar compounds including bisphenol F (BPF) or bisphenol S (BPS) even at much higher concentration. Further development of this platform may prove useful in monitoring exposure to BPA and other small molecules in various matrices.

Due to the friendly temperature for virus survival, SARS-CoV-2 is frequently found in cold-chain foods, posing a serious threat to public health. Utilizing an interdigitated microelectrode chip modified with an antibody probe and integrating dielectrophoresis enrichment with interfacial capacitance sensing, a strategy is presented for the detection of trace level spike-protein from SARS-CoV-2. It achieves a limit of detection as low as 2.29 × 10−6 ng/mL in 20 s, with a wide linear range of 10−5–10−1 ng/mL and a selectivity of 234:1. The cost for a single test can be controlled to ~1 dollar. This strategy provides a competitive solution for real-time, sensitive, selective, and large-scale application in cold-chain food quarantine.