Two molecular sensors that specifically recognize ADP in a background of over 100-fold molar excess of ATP are described. These sensors are nucleic-acid based and comprise a general method for monitoring protein kinase activity. The ADP-aptamer scintillation proximity assay is configured in a single-step, homogeneous format while the allosteric ribozyme (RiboReporter) sensor generates a fluorescent signal upon ADP-dependent ribozyme self-cleavage. Both systems perform well when configured for high-throughput screening and have been used to rediscover a known protein kinase inhibitor in a high-throughput screening format.
A 15-minute, single-intervention, individually guided, Vedanta-based meditation was evaluated for acute effects on vital signs and mood indices. This study included 99 volunteers. Subjects were led in meditations incorporating breathing techniques, relaxation exercises, mental imagery, and silent mantra repetition. Pre-and post-intervention vital signs and mood indices were assessed, including heart rate, respiratory rate, blood pressure, temperature, Beck Depression Inventory (BDI), and Likert scales for mood. The meditation was shown to have statistically significant effects in the immediate improvement of vital signs and mood indices. The most substantial effects occurred for blood pressure, respiratory rate, Beck Depression Inventory, depression, anxiousness, happiness, and peacefulness. Also, males experienced greater physiological benefit whereas females experienced greater psychological benefit. The results of this study could be useful in developing gender-specific meditations to improve vital signs and mood indices in acute settings.
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