ADP-Specific Sensors Enable Universal Assay of Protein Kinase Activity

Srinivasan, Jayaram; Cload, Sharon T.; Hamaguchi, Nobuko; Kurz, Jeffrey C.; Keene, Sara; Kurz, Markus; Boomer, Ryan M.; Blanchard, Jill; Epstein, David; Wilson, Charles B.; Diener, John L

doi:10.1016/j.chembiol.2004.03.014

Cited by 85 publications

(64 citation statements)

References 71 publications

(7 reference statements)

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“…To date they have been only sparsely explored in anion recognition, despite examples of metal‐ion binding being quite numerous 73, 74. Aptamers for nucleoside phosphates are well known 75. In particular, an ATP aptamer has been discovered which is highly selective for the longer oligophosphates over the shorter analogues, which shows that nucleic acids can even bind polyanions strongly and selectively (in the presence of Mg 2+ ions) 76.…”

Section: Macromolecular Recognition Of Anions In Watermentioning

confidence: 99%

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective

2015

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The recognition of anions in water remains a key challenge in modern supramolecular chemistry, and is essential if proposed applications in biological, medical, and environmental arenas that typically require aqueous conditions are to be achieved. However, synthetic anion receptors that operate in water have, in general, been the exception rather than the norm to date. Nevertheless, a significant step change towards routinely conducting anion recognition in water has been achieved in the past few years, and this Review highlights these approaches, with particular focus on controlling and using the hydrophobic effect, as well as more exotic interactions such as C−H hydrogen bonding and halogen bonding. We also look beyond the field of small‐molecule recognition into the macromolecular domain, covering recent advances in anion recognition based on biomolecules, polymers, and nanoparticles.

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Section: Macromolecular Recognition Of Anions In Watermentioning

confidence: 99%

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective

2015

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“…21 Other methods have been described that may be suitable but are not currently commercially available. [22][23][24] A key advantage of the PNP + RiboGreen method is its low cost relative to commercial reagents. Commercially supplied polyU and RiboGreen are inexpensive considering the low concentrations required.…”

Section: Discussionmentioning

confidence: 99%

A Homogeneous, High-Throughput-Compatible, Fluorescence Intensity–Based Assay for UDP-N-Acetylenolpyruvylglucosamine Reductase (MurB) with Nanomolar Product Detection

et al. 2012

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“…Therefore, the portfolio of technologies used to screen for kinase inhibitors can be expected to grow further both through the optimization of existing formats, for example, coupled enzyme assays, 36 and through the development and evaluation of novel readouts such as biosensors based on RNA aptamers 37 or nucleotide-converting enzymes. 38 …”

Section: Discussionmentioning

confidence: 99%

Readout Technologies for Highly Miniaturized Kinase Assays Applicable to High-Throughput Screening in a 1536-Well Format

Klumpp¹,

Boettcher²,

Becker³

et al. 2006

SLAS Discovery

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This article discusses the development of homogeneous, miniaturized assays for the identification of novel kinase inhibitors from very large compound collections. In particular, the suitability of time-resolved fluorescence resonance energy transfer (TR-RET) based on phospho-specific antibodies, an antibody-independent fluorescence polarization (FP) approach using metal-coated beads (IMAP™ technology), and the determination of adenosine triphosphate consumption through chemiluminescence is evaluated. These readouts are compared with regard to assay sensitivity, compound interference, reagent consumption, and performance in a 1536-well format, and practical considerations for their application in primary screening or in the identification of kinase substrates are discussed. All of the tested technologies were found to be suitable for miniaturized high-throughput screening (HTS) in principle, but each of them has distinct limitations and advantages. Therefore, the target-specific selection of the most appropriate readout technology is recommended to ensure maximal relevance of HTS campaigns. (Journal of Biomolecular Screening 2006:617-633)

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ADP-Specific Sensors Enable Universal Assay of Protein Kinase Activity

Cited by 85 publications

References 71 publications

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective

Anion Recognition in Water: Recent Advances from a Supramolecular and Macromolecular Perspective

A Homogeneous, High-Throughput-Compatible, Fluorescence Intensity–Based Assay for UDP-N-Acetylenolpyruvylglucosamine Reductase (MurB) with Nanomolar Product Detection

Readout Technologies for Highly Miniaturized Kinase Assays Applicable to High-Throughput Screening in a 1536-Well Format

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