The analytical methods used for herbal analysis are need to be economic, fast and also produce minimum quantities of hazardous chemical waste. Presently analytical community put interest in the research area of non‐hazardous and eco‐friendly practices to develop various green chromatographic methods for routine quality analysis. High cost of phytochemical analysis and uses of hazardous chemicals with high‐end sophisticated instrument, the attempt made to develop a simple analytical method for multiple samples with short time and less uses of solvents. A HPTLC method was developed for simultaneous determination of biological important constituents like β‐sitosterol, taraxerol, clitorienolactone B and β‐sitosterol glycoside from Clitoria ternatea Linn. The proposed method was validated and satisfied the ICH guidelines to demonstrate that the method is adaptable for its intended purpose. The method is simple, sensitive and economic it therefore embraces potential for detection, monitoring, and simultaneous quantification of the four bioactive compounds for C. ternatea and could also be apply to other species.
Evaluation of chemical diversity among genotypes of medicinal plants plays a pivotal role for its improvement and large scale cultivation. Chemical variability of bioactive principles, viz. taraxerol and b-sitosterol are analyzed in 11 populations of Clitoria ternatea L., an important memory enhancer used in Ayurveda. The study revealed high diversity among the collected samples with concentrations (mg/g on dry weight basis) ranging from 0.358±0.006 to 1.04±0.024 mg/g and 0.183±0.004 to 0.334±0.009 mg/g for taraxerol and b-sitosterol, respectively. The chemical variation was examined for different accessions under the same cultivation and environmental conditions during the years 2014 and 2015 at DMAPR, Anand, Gujarat, India. Selection of elite accession was made possible by screening germplasm collected from various parts of India. The results of the experiment refute the popular belief that plants with white flowers are superior to blue flower on phytochemical content basis. Further a validated HPTLC method was developed for the simultaneous determination of taraxerol and b-sitosterol from the species. The method was also applied to study the effect of solvent and plant parts to achieve enhanced active ingredient extract. Results showed that petroleum ether is the most effective solvent for extraction of the two major pharmacologically active constituents, viz. taraxerol (126±4.368 mg/mg of root extract) and b-sitosterol (13.2±1.706 mg/mg of root extract) due to enriched content with high purity root extract of C. ternatea.
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