In
this study, we examined the influence of functionalized poly(3,4-ethylenedioxythiophene)
(PEDOT) nanostructures decorated on the channel layer of an organic
electrochemical transistor (OECT) for the detection of sweat cortisol,
an adrenocorticosteroid stress hormone. The OECT device featured a
bilayer channel confined by a PEDOT:polystyrenesulfonate (PSS) underlayer
and a nanostructure-decorated upper layer engineered from the monomers
EDOT-COOH and EDOT-EG3 through template-free electrochemical polymerization.
This molecular design allowed antibody conjugation using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysulfosuccinimide coupling through the carboxylic
acid side chain, with EDOT-EG3 known to minimize nonspecific binding
of biomolecules. We also engineered an OECT device having a channel
area without any nanostructures to gain insight into the effect of
the nanostructures on cortisol sensing. Our new nanostructure-embedded
OECT device facilitated real-time detection of cortisol at concentrations
ranging from 1 fg/mL to 1 μg/mL with a detection limit of 0.0088
fg/mL with good linearity (R
2 = 0.9566),
in addition to excellent selectivity toward cortisol among other structurally
similar interfering compounds and high stability and reproducibility.
With its rapid response for the detection of 100 ng/mL cortisol-spiked
artificial sweat, this nanostructure-decorated OECT device has potential
clinical practicality and utility in wearable sensors for future healthcare
applications.
In this study we investigated the synergistic effects of the chirality (molecular structure) and surface morphology (nanostructure) of a newly designed sensing platform for the stereoselective recognition of biomolecules. We...
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