Fast and reliable propagation of plant material is an important need in different stages of breeding programs and production systems. In vitro propagation ensures that pathogen- and virus-free plants stay phytosanitary clean over time while providing high multiplication rates. Using liquid instead of solid culture medium can reduce the interval of individual propagation cycles and contributes to speeding up of the process (1.5–2.5 times), especially during the initial growth phase of the plants. Sophisticated immersion systems have been developed for many plant species, but they are difficult to apply when hundreds or thousands of different genotypes are propagated simultaneously. Additionally, these systems require a high input of technical equipment, know-how and experience to avoid bacterial or fungal contamination during the propagation process. The following protocol describes a low-input suspension technique that combines the use of liquid and solid medium, and permits the successful propagation of genetically diverse sweetpotato genotypes [Ipomoea batatas (L.) Lam.] with a high multiplication rate. As sweetpotato is an important staple crop in low-income/technology countries of Africa, Asia, and South America, the described method may find valuable application for the breeding programs in these regions.
Resumen Las Callas (Zantedeschia spp.) son plantas de la familia Araceae de gran interés como plantas ornamentales en maceteros y como flores de corte. Resulta de gran interés la propagación in vitro de las nuevas variedades obtenidas, por la diversidad de colores de sus flores. Cuando se desean introducir nuevos híbridos en el mercado, las técnicas de propagación tradicional son insuficientes, por lo que resultan de gran interés las técnicas de propagación in vitro. Con el objetivo de establecer un protocolo para la propagación in vitro más eficiente se evaluaron diferentes formas de cultivo (semi-sólido; líquido en movimiento e inmersión temporal), en la proliferación y calidad de los brotes. Se logró el establecimiento de un protocolo para la multiplicación in vitro de la Zantedeschia en Sistemas de Inmersión Temporal de mayor eficiencia biológica que los métodos convencionales de propagación (semi-sólido y liquido en movimiento).Palabras claves: Zantedeschia, medio de cultivo, micropropagación, ornamentales. AbstRActC allas (Zantedeschia spp.) belonging to Araceae family are ornamental plants with high commercial demand as plants in pot and cut flower. Traditional propagation techniques are not able to satisfy the fast introduction of new hybrids to the market. Thus, in vitro culture techniques are a useful tool for the propagation of new varieties with a wide range of colours. Aiming to establish a more efficient protocol for the in vitro propagation of callas, the effect of the way of culture (semisolid, shaken liquid media and Temporary Immersion) on shoots proliferation and quality, were evaluated. The protocol for in vitro propagation of Zantedeschia involving Temporary Immersion Systems showed higher biological efficiency than those through conventional methods (semi-solid and shaken liquid media).
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