ObjectiveTo compare embryo quality, fertilization, implantation, miscarriage and
clinical pregnancy rates for embryos cultured in two different commercial
culture media until D-2 or D-3.MethodsIn this retrospective study, we analyzed 189 cycles performed in 2016.
Metaphase II oocytes were microinjected and allocated into single medium
(SAGE 1-STEP, Origio) until transferred, frozen or discarded; or, if
sequential media were used, the oocytes were cultured in G1-PLUS™
(Vitrolife) up to D-2 or D-3 and in G2-PLUS™ (Vitrolife) to transfer.
On the following day, the oocytes were checked for normal fertilization and
on D-2 and D-3 for morphological classification. Statistical analysis was
performed using the chi-square and Mann-Whitney tests in PASW Statistics
18.0.ResultsThe fertilization rates were 70.07% for single and 69.11% for sequential
media (p=0.736). The mean number of embryos with high
morphological quality (class A/B) was higher in the single medium than in
the sequential media: D-2 [class A (190 vs. 107,
p<0.001), B (133 vs. 118, p=0.018)];
D-3 [class A (40 vs. 19, p=0.048) but without differences
in class B (40 vs. 49)]. Consequently, a higher number of embryos cultured
in single medium were frozen: 197 (21.00%) vs. sequential: 102 (11.00%),
p<0.001. No differences were found in implantation
rates (30.16% vs. 25.57%, p=0.520), clinical pregnancy
rates (55.88% vs. 41.05%, p=0.213), or miscarriage rates
(14.29% vs. 9.52%, p=0.472).ConclusionEmbryo culture in single medium yields greater efficiency per cycle than in
sequential media. Higher embryo quality and quantity were achieved,
resulting in more frozen embryos. There were no differences in clinical
pregnancy rates.
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