In the present work, we have developed a set of sequential and complementary alternatives to valorise five fish discards in order to recover and produce fish mince, oils, gelatins, fish protein hydrolysates (FPHs) with antioxidant and antihypertensive properties and marine peptones. In this last case, microbial bioconversion of marine peptones was studied by Pediococcus acidilacti growth, a well-known valuable lactic acid bacterium, and the concomitant production of lactic acid and pediocin SA-1. Highly digestible FPHs with a good quality of amino acids composition and antioxidant and antihypertensive properties were easily produced. In the same bioprocess, fish oils with a healthy ω-3/ ω-6 ratio were also isolated. The use of peptones derived from FPHs as bacterial media nutrient (source of organic nitrogen) led to reduction of bioproductions costs between 2-5 folds in comparison with commercial culture medium.
A new proteolytic activity assay was devised to avoid the interference of paramyosin which causes gelling during the enzymatic assay. Extremely high autolytic activity was observed in octopus arm muscle, which was 40–500 fold higher than those of various other fish species. The proteinase was inhibited strongly by leupeptin and iodoacetic acid and, to a lesser degree, by transepoxysuccinyl‐L‐leucylamino (4‐guanidono)butane (E‐64), indicating the class as a thioi proteinase. The proteinase exhibited optimum activity at pH 2.5 and 40C, although it contained a sulfhydryl group in the active site. Myosin heavy chain was the primary myofibrillar protein which was hydrolyzed during the autolysis of octopus arm followed by paramyosin. Actin showed no signs of hydrolysis during the incubation of up to 8 h. Due to its high affinity for myosin, the enzyme activity should be controlled during processing octopus to ensure the functionality of myosin.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.