The microorganisms play a vital role in fertility of soil and hence favors' large group of plants. In present study we have successfully isolated certain bacteria from soil including both Gram positive and Gram negative bacteria and access their ability in phosphate solubilization. Effects of temperature, pH and different carbon sources, on phosphate solubilization, by these isolates were also determined. Low temperature 25 °C, pH between 5-7, and glucose as carbon source were found to be best for phosphate solubilization by most of the isolates. Present study highlights the importance of these plants growth promoting bacteria and their uses for agriculture purposes.
Diabetes is one of the major health issues globally. Type 1 diabetes mellitus develops due to the destruction of pancreatic β cells. Mesenchymal stem cells (MSCs) having remarkable self‐renewal and differentiation potential, can regenerate β cells. MSCs preconditioned with bioactive small molecules possess enhanced biological features and therapeutic potential under in vivo environment. Interestingly, compounds of naphthoquinone class possess antidiabetic and anti‐inflammatory properties, and can be explored as potential candidates for preconditioning MSCs. This study analyzed the effect of lawsone‐preconditioned human umbilical cord MSCs (hUMSCs) on the regeneration of β cells in the streptozotocin (STZ)‐induced Type 1 diabetes (T1D) rats. hUMSCs were isolated and characterized for the presence of surface markers. MSCs were preconditioned with optimized concentration of lawsone. T1D rat model was established by injecting 50 mg/kg of STZ intraperitoneally. Untreated and lawsone‐preconditioned hUMSCs were transplanted into the diabetic rats via tail vein. Fasting blood sugar and body weight were monitored regularly for 4 weeks. Pancreas was harvested and β cell regeneration was evaluated by hematoxylin and eosin staining, and gene expression analysis. Immunohistochemistry was also done to assess the insulin expression. Lawsone‐preconditioned hUMSCs showed better anti‐hyperglycemic effect in comparison with untreated hUMSCs. Histological analysis presented the regeneration of islets of Langerhans with upregulated expression of βcell genes and reduced expression of inflammatory markers. Immunohistochemistry revealed strong insulin expression in the preconditioned hUMSCs compared with the untreated hUMSCs. It is concluded from the present study that lawsone‐preconditioned hMSCs were able to exhibit pronounced anti‐hyperglycemic effect in vivo compared with hUMSCs alone.
Background: Diabetes is one of the major health issues globally. T1DM
develops due to the destruction of pancreatic beta cells. Mesenchymal
stem cells having remarkable self-renewal and differentiation potential,
can regenerate beta cells. MSCs preconditioned with bioactive small
molecules possess enhanced biological features and therapeutic potential
under in vivo environment. Interestingly, compounds of naphthoquinone
class possess anti-diabetic, anti-inflammatory properties, and can be
explored as potential candidates for preconditioning MSCs. Aim: This
study analyzed the effect of lawsone preconditioned human umbilical cord
MSCs (hUMSCs) on the regeneration of β-cells in the STZ-induced type 1
diabetic rats. Methods: hUMSCs were isolated and characterized for the
presence of surface markers. MSCs were preconditioned with optimized
concentration of lawsone. T1D rat model was established by injecting 50
mg/kg of streptozotocin intraperitoneally. Untreated and lawsone
preconditioned MSCs were transplanted into the diabetic rats via tail
vein. Fasting blood sugar and body weight were monitored regularly for 4
weeks. Pancreas was harvested and beta cell regeneration was evaluated
by H&E staining and gene expression analysis. Immunohistochemistry was
also done to assess the insulin protein expression. Results: Lawsone
preconditioned MSCs showed better anti-hyperglycemic effect, compared to
untreated MSCs. Histological analysis presented the regeneration of
islets of Langerhans with higher expression of β-cell genes and reduced
expression of inflammatory markers. Immunohistochemistry revealed
intense insulin expression in the preconditioned compared to the
untreated MSCs. Conclusion: It is concluded from the present study that
lawsone preconditioned MSCs were able to exhibit pronounced
anti-hyperglycemic effect in vivo compared to MSCs alone.
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