The aim of the current study was to explore the antioxidant potential and cytotoxicity of different 'fractions from the leaf extract of Randia dumetorum. Proximate analysis and phytochemical screening were done with standard protocol. The antioxidant activity was evaluated by using reducing power assay, total antioxidant capacity determination, determination of total phenolic content, determination of total flavonoid content and reduction of ferric ions by ortho-phenanthroline color method. In the antioxidant studies, ascorbic acid, gallic acid and butylated hydroxy toluene (BHT) were used as standard antioxidant compound. The brine shrimp lethality test was used to examine cytotoxicity. In the proximate analysis, moisture content, total ash value, acid insoluble ash and water-soluble ash value were found in the leaf of R. dumetorum. Concerning the phytochemical screening, there was the presence of glycosides, flavonoids, reducing sugars, saponins, phenolic compounds, tannins on different fractions, but the absence of gums and mucilage, alkaloid, protein, and amino acid. The results show that all the extracts of R. dumetorum leaf possess significant antioxidant activity. 'The methanol and water extracts of the leaves showed significant cytotoxic activity as compared to dimethyl sulfoxide (DMSO) (LC 50 1.07 µg/ml). These findings suggest that R. dumetorum leaves might be a good antioxidants source and possess mild cytotoxic effect.