Apolipoprotein B (apoB) and microsomal triglyceride transfer protein (MTP) are necessary for lipoprotein assembly. ApoB consists of five structural domains, ␣ 1 - 1 -␣ 2 - 2 -␣ 3 . We propose that MTP contains three structural motifs (N-terminal  -barrel, central ␣ -helix, and C-terminal lipid cavity) and three functional domains (lipid transfer, membrane associating, and apoB binding). MTP's lipid transfer activity is required for the assembly of lipoproteins. This activity renders nascent apoB secretion-competent and may be involved in the import of triglycerides into the lumen of endoplasmic reticulum. In addition, MTP binds to apoB with high affinity involving ionic interactions. MTP interacts at multiple sites in the N-terminal ␣ 1 structural domain of apoB. A novel antagonist that inhibits apoB-MTP binding decreases apoB secretion. Furthermore, site-directed mutagenesis and deletion analyses that inhibit apoB-MTP binding decrease apoB secretion. Lipids modulate protein-protein interactions between apoB and MTP. Lipids associated with MTP increase apoB-MTP binding whereas lipids associated with apoB decrease this binding. Thus, specific antagonist, site-directed mutagenesis, deletion analyses, and modulation studies support the notion that apoB-MTP binding plays a role in lipoprotein biogenesis. However, specific steps in lipoprotein assembly that require apoB-MTP binding have not been identified.ApoB-MTP binding may be important for the prevention of degradation and lipidation of nascent apoB. Plasma lipoproteins are absent in abetalipoproteinemia due to mutations in the microsomal triglyceride transfer protein (MTP) gene, and plasma lipoprotein levels are low in hypobetalipoproteinemia due to mutations in the apolipoprotein B (apoB) gene (1, 2). These genetic disorders clearly underscore the importance of these two proteins in lipoprotein biogenesis, and recent findings indicate that MTP and apoB physically interact during this process. The aim of this review is to discuss specific molecular interactions between these proteins and their role in the biosynthesis of triglyceride-rich lipoproteins. A brief review of apoB and MTP is provided to aid in the understanding of protein-protein interactions between these proteins. In-depth discussion of apoB, MTP, and lipoprotein assembly can be found in several recent reviews and references therein (3-14). STRUCTURAL AND FUNCTIONAL DOMAINS IN APOBApolipoprotein B (apoB) is a non-exchangeable apolipoprotein found associated exclusively with plasma lipoproteins. In the human genome there is one apob gene of ف 45 kb. In the liver, it is transcribed into a single mRNA of 15 kb and is translated into a single polypeptide of 4536 amino acids called apoB-100. In the intestine, the apoB mRNA is post-transcriptionally edited, resulting in the conversion of a glutamine codon into a stop codon. The edited mRNA is translated into a single polypeptide of 2,152 amino acids called apoB48. By comparing mean hydrophobic moments per amino acid residue and the average...
Higher aridity and more extreme rainfall events in drylands are predicted due to climate change. Yet, it is unclear how changing precipitation regimes may affect nitrogen (N) cycling, especially in areas with extremely high aridity. Here we investigate soil N isotopic values (d 15 N) along a 3,200 km aridity gradient and reveal a hump-shaped relationship between soil d 15 N and aridity index (AI) with a threshold at AI ¼ 0.32. Variations of foliar d 15 N, the abundance of nitrification and denitrification genes, and metabolic quotient along the gradient provide further evidence for the existence of this threshold. Data support the hypothesis that the increase of gaseous N loss is higher than the increase of net plant N accumulation with increasing AI below AI ¼ 0.32, while the opposite is favoured above this threshold. Our results highlight the importance of N-cycling microbes in extremely dry areas and suggest different controlling factors of N-cycling on either side of the threshold.
Soil microbial communities are extremely complex, being composed of thousands of low-abundance species (<0.1% of total). How such complex communities respond to natural or human-induced fluctuations, including major perturbations such as global climate change, remains poorly understood, severely limiting our predictive ability for soil ecosystem functioning and resilience. In this study, we compared 12 whole-community shotgun metagenomic data sets from a grassland soil in the Midwestern United States, half representing soil that had undergone infrared warming by 2°C for 10 years, which simulated the effects of climate change, and the other half representing the adjacent soil that received no warming and thus, served as controls. Our analyses revealed that the heated communities showed significant shifts in composition and predicted metabolism, and these shifts were community wide as opposed to being attributable to a few taxa. Key metabolic pathways related to carbon turnover, such as cellulose degradation (ϳ13%) and CO 2 production (ϳ10%), and to nitrogen cycling, including denitrification (ϳ12%), were enriched under warming, which was consistent with independent physicochemical measurements. These community shifts were interlinked, in part, with higher primary productivity of the aboveground plant communities stimulated by warming, revealing that most of the additional, plant-derived soil carbon was likely respired by microbial activity. Warming also enriched for a higher abundance of sporulation genes and genomes with higher G؉C content. Collectively, our results indicate that microbial communities of temperate grassland soils play important roles in mediating feedback responses to climate change and advance the understanding of the molecular mechanisms of community adaptation to environmental perturbations.
BackgroundBacteriophage–prokaryote dynamics and interaction are believed to be important in governing microbiome composition and ecosystem functions, yet our limited knowledge of the spatial and temporal variation in phage and prokaryotic community compositions precludes accurate assessment of their roles and impacts. Anaerobic digesters are ideal model systems to examine phage–host interaction, owing to easy access, stable operation, nutrient-rich environment, and consequently enormous numbers of phages and prokaryotic cells.ResultsEquipped with high-throughput, cutting-edge environmental genomics techniques, we examined phage and prokaryotic community composition of four anaerobic digesters in full-scale wastewater treatment plants across China. Despite the relatively stable process performance in biogas production, phage and prokaryotic groups fluctuated monthly over a year of study period, showing significant correlations between those two groups at the α- and β-diversity levels. Strikingly, phages explained 40.6% of total variations of the prokaryotic community composition, much higher than the explanatory power by abiotic factors (14.5%). Consequently, phages were significantly (P < 0.010) linked to parameters related to process performance including biogas production and volatile solid concentrations. Association network analyses showed phage–prokaryote pairs were shallowly conserved since they were detected only within small viral clades.ConclusionsThose results collectively demonstrate phages as a major biotic factor in controlling prokaryotic composition and process performance. Therefore, phages may play a larger role in shaping prokaryotic community dynamics and process performance of anaerobic digesters than currently appreciated.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-017-0272-8) contains supplementary material, which is available to authorized users.
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