Detailed measurements of physical, chemical and biological dynamics in groundwater are key to understanding the important processes in place and their influence on water quality – particularly when used for drinking water. Measuring temporal bacterial dynamics at high frequency is challenging due to the limitations in automation of sampling and detection of the conventional, cultivation-based microbial methods. In this study, fully automated online flow cytometry was applied in a groundwater system for the first time in order to monitor microbial dynamics in a groundwater extraction well. Measurements of bacterial concentrations every 15 minutes during 14 days revealed both aperiodic and periodic dynamics that could not be detected previously, resulting in total cell concentration (TCC) fluctuations between 120 and 280 cells μL−1. The aperiodic dynamic was linked to river water contamination following precipitation events, while the (diurnal) periodic dynamic was attributed to changes in hydrological conditions as a consequence of intermittent groundwater extraction. Based on the high number of measurements, the two patterns could be disentangled and quantified separately. This study i) increases the understanding of system performance, ii) helps to optimize monitoring strategies, and iii) opens the possibility for more sophisticated (quantitative) microbial risk assessment of drinking water treatment systems.

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Karst aquifers provide drinking water for 10% of the world's population, support agriculture, groundwater-dependent activities, and ecosystems. These aquifers are characterised by complex groundwater-flow systems, hence, they are extremely vulnerable and protecting them requires an in-depth understanding of the systems. Poor data accessibility has limited advances in karst research and realistic representation of karst processes in large-scale hydrological studies. In this study, we present World Karst Spring hydrograph (WoKaS) database, a community-wide effort to improve data accessibility. WoKaS is the first global karst springs discharge database with over 400 spring observations collected from articles, hydrological databases and researchers. The dataset's coverage compares to the global distribution of carbonate rocks with some bias towards the latitudes of more developed countries. WoKaS database will ensure easy access to a large-sample of good quality datasets suitable for a wide range of applications: comparative studies, trend analysis and model evaluation. This database will largely contribute to research advancement in karst hydrology, supports karst groundwater management, and promotes international and interdisciplinary collaborations. Background & Summary Karst aquifers are essential sources of drinking water to about 10% of the world's population 1. In many regions across the globe, karst groundwater is also an indispensable resource for ecosystems, agriculture and, economic activities, as well as for tourism and recreation 2,3. For example, in Europe, 21.6% of the land surface is underlain by carbonate rock 4 which contributes up to 50% of supplied drinking water in some countries 5-7. However, groundwater flow in karst aquifers is characterised by a complex interplay of fast-flowing conduit and slow-flowing matrix systems 8,9. Hence, the storage capacity of karst aquifers is variable and systems are extremely vulnerable to climatic pressures, human impacts and contamination 10. In order to ensure adequate protection of karst water sources, in-depth hydrogeological knowledge is necessary. Large-scale modelling and comparative water resource research have shown the great value of large datasets in hydrology 11. Numerous studies have applied these large datasets for several purposes such as model evaluation, global parameter estimations, impact studies, statistical and comparative analyses. For instance, large-scale hydrological models such as WaterGAP 12 used discharge data from the Global Runoff Data Centre (https://www. bafg.de/GRDC) for parameter estimation. Likewise, streamflow data from the Model Parameter Estimation Experiment (MOPEX) 13 and the Global Runoff Data Centre (GDRC) were combined to derive global base flow indexes and recession constants 14. Streamflow observations of near-natural catchments obtained from UNESCO's European Water Archive (EWA) were used to investigate the streamflow trends across Europe and differentiated the impacts from climatic variability and anthropogenic dri...

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This study presents the development of tools for the sustainable thermal management of a shallow unconsolidated urban groundwater body in the city of Basel (Switzerland). The concept of the investigations is based on (1) a characterization of the present thermal state of the urban groundwater body, and (2) the evaluation of potential mitigation measures for the future thermal management of specific regions within the groundwater body.

The investigations focus on thermal processes down-gradient of thermal groundwater use, effects of heated buildings in the subsurface as well as the thermal influence of river–groundwater interaction. Investigation methods include (1) short- and long-term data analysis, (2) high-resolution multilevel groundwater temperature monitoring, as well as (3) 3-D numerical groundwater flow and heat transport modeling and scenario development. The combination of these methods allows for the quantifying of the thermal influences on the investigated urban groundwater body, including the influences of thermal groundwater use and heated subsurface constructions. Subsequently, first implications for management strategies are discussed, including minimizing further groundwater temperature increase, targeting "potential natural" groundwater temperatures for specific aquifer regions and exploiting the thermal potential

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