No abstract

The present study was conducted to investigate the effects of dietary fiber source levels on the fecal ammonia nitrogen, growth performance, carcass traits, gastrointestinal tract development, and intestinal morphology of broilers. A total of 420 one-day-old unsexed broiler chicks were individually weighed and randomly divided into 5 groups, each with seven replicates of twelve chicks. Rice hulls (RH) and soybean hulls (SH) were ground through a hammer mill with a 2-mm screen. The RH and SH experimental diets were as follows: 0% (control); 2.5% RH; 2.5% SH; 5% RH; and 5% SH. No significant differences were found in growth performance and fecal ammonia nitrogen among the dietary treatment groups (p>0.05). Compared with the control, the experimental diets with 2.5% SH significantly decreased the wing weight of chickens (p<0.05), while no significant differences in the weight of the other visceral organs were observed. Compared with the control, broilers in the 5% SH group had a longer jejunum and ileum (p<0.05). Feeding the broilers SH and RH had no effect on the villus area and crypt depth of the intestine. Compared with the control, the experimental diet with 2.5% RH significantly increased the duodenal villus height of chickens (p<0.05). These findings suggest that the inclusion of 5% SH in the diets resulted in improved intestinal morphology without negatively affecting growth performance and carcass traits.

The effects of dietary untreated whole-grain paddy rice (WPR) on performance and histological intestinal alterations were investigated in Sanuki Cochin male chicks. At 2 weeks of age, chicks showing similar body weights were randomly divided into 3 groups of 10 birds each. The control group was fed with a basal diet (starter diet: CP 21%, ME 3000 kcal/kg; grower diet: CP 18%, ME 2850 kcal/kg; finisher diet: CP 15%, ME 2800 kcal/kg) and the other groups were fed with the basal diet diluted with WPR at 20% (starter diet: CP 17.8%, ME 2958 kcal/kg; grower diet: CP 15.4%, ME 2838 kcal/kg; finisher diet: CP 13%, ME 2798 kcal/kg) and 40% (starter diet: CP 14.6%, ME 2916 kcal/kg; grower diet: CP 12.8%, ME 2826 kcal/kg; finisher diet: CP 11%, ME 2796 kcal/kg). They were housed in individual cages under natural room temperature (around 5℃) with a daily lighting regimen of 16 h of light and 8 h of dark. The growth performance, relative length of the intestines and relative weight of the visceral organs to 100 g body weight did not differ except that the weight of the gizzard increased significantly (p＜0.05) in the WPR groups. Most parameters of villus height, villus area, cell area and cell mitosis numbers of the WPR groups did not show a significant decrease. In scanning electron microscopic results, the morphology of the villus apical surface in the WPR groups did not show damage due to WPR and had similar cells to the control (protuberated cells). These results demonstrate that WPR can be diluted by up to 40% as a feed ingredient in chicken basal diets.

The present study was conducted to investigate replacing corn with whole-grain paddy rice (WPR) and whole-grain brown rice (BR) in broiler chicken diets and its effect on growth performance and histological structures of the intestinal villi. Marshall Chunky male chicks (14 days old) were divided into five groups with four replicates of four chicks each. In the dietary treatments, corn in the basal diet was replaced with WPR and BR. The chickens received five experimental diets consisting of corn, WPR and BR in ratios of 100:0:0 (Control), 50:0:50 (50Corn + 50BR), 50:25:25 (50Corn + 25WPR + 25BR), 25:50:25 (25Corn + 50WPR + 25BR) and 0:50:50 (50WPR + 50BR) respectively. Feed and water were provided ad libitum for 35 day. No significant differences were found in feed intake, body weight gain and feed efficiency among the treatment groups. The relative weights of the gizzard in the 50Corn + 25WPR + 25BR, 25Corn + 50WPR + 25BR and 50WPR + 50BR groups were significantly higher than that of the Control and 50Corn + 50BR groups (p < 0.05). The gizzard pH of the experimental groups was lower than those of Control (p < 0.05). The ileal crypt of birds on the Control diets was deeper (p < 0.05) than those observed in the experimental birds. Moreover, the ileal villus height: crypt depth ratio increased (p < 0.05) in the 50WPR + 50BR group (p < 0.05) compared with the Control group. No specific changes were observed in the epithelial cells on the duodenal apical surface among the groups except that the villus of the 25Corn + 50WPR + 25BR group had cell clusters. The jejunal and ileal villus apical surface of the experimental groups showed similar morphology to the Control group. These findings suggest that WPR and BR can totally replace corn in broiler diets without negatively affecting growth performance.

Two experiments were conducted to investigate the effect of replacing corn with whole-grain paddy rice (WPR) in laying hen diets on egg production performance and quality. Commercial layers (Sonia) were used in both Experiment 1 and 2. In Experiment 1, 80 layers were placed into 4 groups of 20 birds each: the corn in the basal diet was replaced with 0, 10, 30 and 50%WPR. Each group of 20 birds had 10 replicates of 2 birds. In Experiment 2, 45 layers were placed into 3 groups of 15 birds each: the corn in the basal diet was replaced with 0, 70 and 100%WPR. Egg production was recorded daily and feed consumption was measured weekly throughout the experiments. Eggs from each group were collected biweekly to measure egg quality. Egg production performance and quality were not different among the groups (P>0.05), except for a decreased (P<0.05) shell ratio in the 100%WPR group. Moreover, yolk color score decreased (P<0.05) with increasing levels of WPR (50%WPR or more). The present results reveal that WPR can replace up to 100% of corn in laying hen diets without harming egg production performance and quality.

To investigate the effect of replacing maize with whole-grain paddy rice (WPR) in broiler chicken diets, with or without enzyme addition, on growth performance and histological structures of the intestinal villi, 14-d-old Marshall Chunky male chicks were divided into 4 groups with 4 replicates of 4 chicks each. The experimental diets containing different concentrations of WPR were as follows: (1) 0 g/kg (Control); (2) 141.5 g/kg, grower, and 125.0 g/kg, finisher (25WPR); (3) 283.0 g/kg, grower, and 250.0 g/kg, finisher (50WPR); (4) 283.0 g/kg, grower, and 250.0 g/kg, finisher, and enzyme supplementation (50WPR + enzyme). All diets were formulated to be isocaloric and isonitrogenous and provided ad libitum for 35 d. There were no differences among the diets on the growth performance and digestive organ size. The villus height and cell mitosis number of all intestinal segments did not change in any treatment. The ileal villus area, duodenal cell area, duodenal and jejunal goblet cell number in the 50WPR group increased significantly relative to the control but not when enzyme was included. In the scanning electron microscope results, all experimental groups showed clear protuberant cells and cell clusters on the villus apical surface of the duodenum. In the jejunum, cell clusters and areas having cells with no microvilli were frequently found in both the 50WPR and 50WPR + enzyme groups. In conclusion, broilers fed on diets replacing maize with WPR showed hypertrophied villi of duodenum and ileum and epithelial cells in duodenum and jejunum, especially in the 50WPR group, without negatively affecting growth performance. These findings suggest that WPR can replace maize up to a level of 50% (283.0 g/kg, starter, and 250.0 g/kg, finisher) in broiler diets without enzyme supplementation. However, further studies are needed to improve our knowledge of the influence of WPR on higher numbers of birds.

The effect of dietary bark charcoal containing vinegar liquid (BCV) on the growth performance, carcass and visceral organs, intestinal histology, fecal ammonia nitrogen, and nutrient digestibility was investigated in a total of 350 one-day-old unsexed Ross 308 broilers raised under a high environmental temperature (heat stress) from 0 to 42 days of age. They were divided into 0% (control group), 1%, 2%, 3% and 4% dietary BCV groups. Compared with the control group, the body weight gain (BWG) of the 1% dietary BCV group at 21-42 days of age and the feed efficiency of the 2% dietary BCV group at 0-42 days of age increased (P < 0.05). The carcasses of the 1% and 3% dietary BCV groups significantly decreased (P < 0.05). The digestibility of dry matter and gross energy of the 4% dietary BCV group improved at 21 d (P < 0.05). The duodenum of the 3% and 4% dietary BCV groups was significantly heavier than those in the control groups (P < 0.05). This result suggests that BCV can shorten the time to shipping date for broilers to before 42 days of age and can contribute to the development of poultry production in tropical regions.

To determine whether dried fermented ginger (DFG), fermented with Japanese mugwort silage juice, could be replaced by fermented corncob powder (FCP) as a of feed ingredient source without significant body weight decrease or damage to visceral organs (using gross anatomical observation), to intestinal villi (using light microscopy), or to the epithelial cells on the villus apical surface (using scanning electron microscopy) the following investigation was performed. Sixty-four male broilers were allotted to 4 groups: a basal diet group (control group), and basal diet groups with DFG at a level of 50 ppm; with DFG at 50 ppm and FCP at 250 ppm (50 ppm DFG + 250 ppm FCP group); and with FCP at a level of 500 ppm (500 ppm FCP group). Feed intake, body weight gain, feed efficiency, carcass quality, small intestinal length and weight, and visceral organ weight were not different among groups. Furthermore, regarding intestinal villus height, villus area and crypt depth, a significant difference was not found among the groups. When these values of the control were expressed as an index of 100, the duodenal villus height of the 50 ppm DFG + 250 ppm FCP group and the 500 ppm FCP group were 114 and 119, respectively. The duodenal villus area of the 50 ppm DFG + 250 ppm FCP group and the 500 ppm FCP group were 125 and 158, respectively. These villus heights and areas are thought to be activated. On the epithelial cells on the villus apical surface in the duodenum and jejunum, the 50 ppm DFG + 250 ppm FCP group had protuberated cells into the intestinal lumen and deeper cells at the sites of recently exfoliated cells, suggesting that these cells are activated. The present results indicate that small amounts of fermented corncob powder can be used as a feed supplement when mixed with fer- 566mented ginger powder, due to the synergy between the two ingredients, resulting in a 6% increase in body weight gain.