The synthesis of juvenile hormone in vitro by diapausing and nondiapausing Culex pipiens L. was measured by radiochemical assay. Paired corpora allata from diapausing females synthesized < 18 fmols of juvenile hormone per hour during the first 3 wk after emergence. In contrast, juvenile hormone synthesis in nondiapausing females increased rapidly reaching a peak of 87.3 +/- 21 (mean +/- SE) fmol/h 3 d after emergence. By 3 wk, juvenile hormone synthesis had decreased in both groups of females, but corpora allata from nondiapausing mosquitoes still were 3 times more active than those from diapausing mosquitoes. By 16 wk after diapause induction, females maintained at 8:16 (L:D) h and 15 degrees C produced levels of juvenile hormone similar to 3-wk-old nondiapausing females. When females were held in diapause conditions for up to 22 wk, follicles gradually grew longer and by 15 wk were significantly longer than in the previous 14 wk. Blood feeding also increased in older females, indicating that over time, juvenile hormone synthesis gradually stimulated blood-feeding behavior. When 21-d-old diapausing mosquitoes were moved to a long-day photoperiod of 16:8 (L:D) h and 26 degrees C, juvenile hormone synthesis increased rapidly and peaked 5 d later while the ovarian follicles grew to the resting stage. Allatectomy of young diapausing females prevented follicle growth and blood feeding when diapause was terminated prematurely, demonstrating that the physiological events associated with diapause termination were associated with juvenile hormone biosynthesis.

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The efficacy of a photostable formulation of methoprene and two photostable juvenoids, fenoxycarb and pyriproxyfen, and their residual activity in inhibiting the emergence of adult cat fleas, Ctenocephalides felis (Bouché), was studied in topsoil. Nursery pots composed of clay, peat, and plastic, and wooden flats were used to hold soil samples. Treated soil samples were exposed to sunlight during the 63-d study period. Methoprene was as effective as fenoxycarb and pyriproxyfen against cat fleas for up to 42 d in clay, peat, and plastic pots at a concentration of 64.56 mg (AI)/m2 (6 mg [AI]/ft2), but its activity declined significantly thereafter. In contrast, fenoxycarb and pyriproxyfen showed strong residual activity for the entire 63 d. The activity of methoprene declined even more rapidly over time in wooden flats, while at the same concentrations the other two juvenoids showed significant residual activity for 63 d. Clay, peat, and plastic pots were therefore considered to be equally effective for evaluating the outdoor efficacy of juvenoids in comparison to the wooden flats. However, results obtained with wooden flats may be more realistic when testing residual activity of volatile chemicals such as methoprene. Fenoxycarb and pyriproxyfen showed strong efficacy and residual activity at concentrations of 8.07, 16.14, and 32.28 mg (AI)/m2, whereas methoprene did not cause a significant reduction of adult emergence at levels below 64.56 mg (AI)/m2. LC50 values for methoprene, fenoxycarb, and pyriproxyfen needed for preventing flea emergence when applied to topsoil were estimated to be 0.643, 0.031, and 0.028 ppm, respectively.