The human gut harbors a vast range of microbes that have significant impact on health and disease. Therefore, gut microbiome profiling holds promise for use in early diagnosis and precision medicine development. Accurate profiling of the highly complex gut microbiome requires DNA extraction methods that provide sufficient coverage of the original community as well as adequate quality and quantity. We tested nine different DNA extraction methods using three commercial kits (TianLong Stool DNA/RNA Extraction Kit (TS), QIAamp DNA Stool Mini Kit (QS), and QIAamp PowerFecal DNA Kit (QP)) with or without additional bead-beating step using manual or automated methods and compared them in terms of DNA extraction ability from human fecal sample. All methods produced DNA in sufficient concentration and quality for use in sequencing, and the samples were clustered according to the DNA extraction method. Inclusion of bead-beating step especially resulted in higher degrees of microbial diversity and had the greatest effect on gut microbiome composition. Among the samples subjected to bead-beating method, TS kit samples were more similar to QP kit samples than QS kit samples. Our results emphasize the importance of mechanical disruption step for a more comprehensive profiling of the human gut microbiome.
In this paper, we successfully separated malignant human breast cancer epithelial cells (MCF 7) from healthy breast cells (MCF 10A) and analyzed the main parameters that influence the separation efficiency with an advanced dielectrophoresis (DEP)-activated cell sorter (DACS). Using the efficient DACS, the malignant cancer cells (MCF 7) were isolated successfully by noninvasive methods from normal cells with similar cell size distributions (MCF 10A), depending on differences between their material properties such as conductivity and permittivity, because our system was able to discern the subtle differences in the properties by generating continuously changed electrical field gradients. In order to evaluate the separation performance without considering size variations, the cells collected from each outlet were divided into size-dependent groups and counted statistically. Following that, the quantitative relative ratio of numbers between MCF 7 and MCF 10A cells in each size-dependent group separated by the DEP were compared according to applied frequencies in the range 48, 51, and 53 MHz with an applied amplitude of 8 V(pp). Finally, under the applied voltage of 48 MHz-8 V(pp) and a flow rate of 290 microm/s, MCF 7 and MCF 10A cells were separated with a maximum efficiency of 86.67% and 98.73% respectively. Therefore, our suggested system shows it can be used for detection and separation of cancerous epithelial cells from noncancerous cells in clinical applications.
Thyroid hormone plays an essential role in myogenesis, the process required for skeletal muscle development and repair, although the mechanisms have not been established. Skeletal muscle develops from the fusion of precursor myoblasts into myofibers. We have used the C2C12 skeletal muscle myoblast cell line, primary myoblasts, and mouse models of resistance to thyroid hormone (RTH) α and β, to determine the role of thyroid hormone in the regulation of myoblast differentiation. T3, which activates thyroid hormone receptor (TR) α and β, increased myoblast differentiation whereas GC1, a selective TRβ agonist, was minimally effective. Genetic approaches confirmed that TRα plays an important role in normal myoblast proliferation and differentiation and acts through the Wnt/β-catenin signaling pathway. Myoblasts with TRα knockdown, or derived from RTH-TRα PV (a frame-shift mutation) mice, displayed reduced proliferation and myogenic differentiation. Moreover, skeletal muscle from the TRα1PV mutant mouse had impaired in vivo regeneration after injury. RTH-TRβ PV mutant mouse model skeletal muscle and derived primary myoblasts did not have altered proliferation, myogenic differentiation, or response to injury when compared with control. In conclusion, TRα plays an essential role in myoblast homeostasis and provides a potential therapeutic target to enhance skeletal muscle regeneration.
We consider scenarios in which we wish to perform joint scene understanding, object tracking, activity recognition, and other tasks in environments in which multiple people are wearing body-worn cameras while a third-person static camera also captures the scene. To do this, we need to establish person-level correspondences across first-and thirdperson videos, which is challenging because the camera wearer is not visible from his/her own egocentric video, preventing the use of direct feature matching. In this paper, we propose a new semi-Siamese Convolutional Neural Network architecture to address this novel challenge. We formulate the problem as learning a joint embedding space for first-and third-person videos that considers both spatial-and motion-domain cues. A new triplet loss function is designed to minimize the distance between correct first-and third-person matches while maximizing the distance between incorrect ones. This end-to-end approach performs significantly better than several baselines, in part by learning the first-and third-person features optimized for matching jointly with the distance measure itself.
Manganese (Mn) is an essential trace element, but excess exposure leads to accumulation in biological tissues, including the brain. Chronically high Mn levels in the brain are neurotoxic and can result in a progressive, irreversible neurological disorder known as manganism. Manganism has signs and symptoms similar to, but distinguishable from idiopathic Parkinson’s disease, which include both psychological and motor disturbances. Evidence suggests that Mn exposure impacts neurotransmitter levels in the brain. However, it remains unclear if subacute, low-level Mn exposure resulted in alterations in neurotransmitter systems with concomitant behavioral deficits. The current study used high performance liquid chromatography to quantify neurotransmitter levels in rat striatum (STR), substantia nigra (SN), and hippocampus (HP). Subacute Mn exposure via i.p. injection of 15 mg Mn/kg as MnCl2 caused significantly increased dopamine (DA) levels in the STR. The enhancement was accompanied by significantly elevated levels of the DA metabolites, 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), in the STR. In addition, levels of HVA were significantly increased in the SN and HP. These data indicate that subacute, low-level Mn exposure disrupts multiple neurotransmitter systems in the rat brain which may be responsible, in part, for observed locomotor deficits.
Oil-free turbochargers (TCs) will increase the power and efficiency of internal combustion engines, both sparking ignition and compression ignition, without engine oil lubricant feeding or scheduled maintenance. Using gas foil bearings (GFBs) in passenger vehicle TCs enables compact, lightweight, oil-free systems, along with accurate shaft motion. This paper presents extensive test measurements on GFBs for oil-free TCs, including static load-deflection measurements of test GFBs, rotordynamic performance measurements of a compressed air driven oil-free TC unit supported on test GFBs, and bench test measurements of the oil-free TC driven by a passenger vehicle diesel engine. Two configurations of GFBs, one original and the other modified with three shims, are subjected to a series of experimental tests. For the shimmed GFB, three metal shims are inserted under the bump-strip layers, in contact with the bearing housing. The installation of shims creates mechanical preloads that enhance a hydrodynamic wedge in the assembly radial clearance to generate more film pressure. Simple static load-deflection tests estimate the assembly radial clearance of the shimmed GFB, which is smaller than that of the original GFB. Model predictions agree well with test data. The discrepancy between the model predictions and test data is attributed to fabrication inaccuracy in the top foil and bump strip layers. Test GFBs are installed into a TC test rig driven by compressed air for rotordynamic performance measurements. The test TC rotor, 335 g in weight and 117 mm long, is coated with a commercially available, wear-resistant solid lubricant, Amorphous M, to prevent severe wear during start-up and shutdown in the absence of an air film. A pair of optical proximity probes positioned orthogonally at the compressor end record lateral rotor motions. Rotordynamic test results show that the shimmed GFB significantly diminishes the large amplitude of subsynchronous rotor motions arising in the unmodified GFB. Predicted synchronous rotor amplitudes and rigid body mode natural frequencies agree reasonably well with recorded test data. Finally, the oil-free TC is installed into a passenger vehicle diesel engine test bench. The TC rotor speed is controlled by the vehicle engine. Speed-up tests show dominant synchronous motion (1X) of the rotor. Whirl frequencies of the relatively small subsynchronous motions are associated with the rigid body natural mode of the TC rotor-GFB system as well as (forced) excitation from the four-cylinder diesel engine. The bench test measurements demonstrate a significant reduction in the amplitude of subsynchronous motions for the shimmed GFB, thus verifying the preliminary test results in the TC test rig driven by compressed air.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
334 Leonard St
Brooklyn, NY 11211
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.